HER3/ErbB3 (1B2E) Rabbit mAb

• 产品编号：CST-4754S      品牌：CST       原厂货号：4754S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HER3/ErbB3 (1B2) 兔单抗，用于检测内源性HER3/ErbB3的总蛋白水平。本抗体与其它ErbB家族成员无交叉反应。单克隆抗体通过用多肽免疫动物得到，该多肽是根据人的ErbB3蛋白Tyr867附近的氨基酸序列合成的。HER3/ErbB3是ErbB酪氨酸激酶受体蛋白家族的成员之一，但却没有酪氨酸激酶活性。ErbB3的酪氨酸位点磷酸化依赖于其它ErbB酪氨酸激酶。与配体结合后，ErbB3与其它ErbB蛋白形成异二聚体,并且其酪氨酸位点能够被激活的ErbB激酶磷酸化（1，2）。在ErbB3的羧基端至少有9个潜在的酪氨酸磷酸化位点。这些位点都是信号转导蛋白一致的结合位点，这些蛋白包括Src家族的分子，Grb2, 和PI3K激酶的p85亚基，它们都能参与ErbB的下游信号调节(3)。ErbB3 的Tyr1222和Tyr1289残基都位于一个YXXM基序上并参与PI3K激酶的信号传递(4)。ErbB3高表达于很多癌细胞中（5），而ErbB3/PI3K通路的激活也和许多腺癌的恶性特征相关（6）。在肿瘤发展过程中，ErbB3可能和ErbB家族的其它成员（如，ErbB2需要和ErbB3一起促进乳腺癌细胞的增殖）作为一个原癌基因单位一起发挥功能（7）。因此，抑制ErbB3和ErbB酪氨酸激酶的相互作用已经成为抗癌治疗中的一个新策略。

1. Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37.
2. Guy, P.M. et al. (1994) Proc Natl Acad Sci U S A 91, 8132-6.
3. Songyang, Z. et al. (1993) Cell 72, 767-78.
4. Kim, H.H. et al. (1994) J Biol Chem 269, 24747-55.
5. Sithanandam, G. et al. (2003) Carcinogenesis 24, 1581-92.
6. Kobayashi, M. et al. (2003) Oncogene 22, 1294-301.
7. Holbro, T. et al. (2003) Proc Natl Acad Sci U S A 100, 8933-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

HER3/ErbB3 (1B2) Rabbit mAb detects endogenous levels of total HER3/ErbB3 proteins. This antibody does not cross-react with other ErbB family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr867 of human ErbB3.

Background

HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3 kinase (4). 
 ErbB3 is highly expressed in many cancer cells (5) and activation of the ErbB3/PI3K pathway is correlated with malignant phenotypes of adenocarcinomas (6). In tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members (e.g. ErbB2 requires ErbB3 to drive breast tumor cell proliferation) (7). Thus, inhibiting interaction between ErbB3 and ErbB tyrosine kinases has become a novel strategy for anti-tumor therapy.

1. Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37.
2. Guy, P.M. et al. (1994) Proc Natl Acad Sci U S A 91, 8132-6.
3. Songyang, Z. et al. (1993) Cell 72, 767-78.
4. Kim, H.H. et al. (1994) J Biol Chem 269, 24747-55.
5. Sithanandam, G. et al. (2003) Carcinogenesis 24, 1581-92.
6. Kobayashi, M. et al. (2003) Oncogene 22, 1294-301.
7. Holbro, T. et al. (2003) Proc Natl Acad Sci U S A 100, 8933-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody