Topoisomerase IIα Antibody

• 产品编号：CST-4733S      品牌：Cell Signaling Technology       原厂货号：4733S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Topoisomerase IIalpha Antibody能够检测内源性Topoisomerase IIalpha总蛋白水平。通过合成的与人源Topoisomerase IIalpha蛋白相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。DNA topoisomerase I和II是细胞核酶，并且type II是由两个高度同源的亚型：topoisomerase IIalpha 和IIbeta。这些酶可调节DNA拓扑结构、维持基因组稳定性和对于例如DNA复制、重组、转录和通过DNA双链分配的染色体分离等过程是必要的(1)。Topoisomerase I分离和重连双螺旋DNA的一条单链，但是topoisomerase II可瞬间中断和关闭双链DNA(2)。Topoisomerases很容易受到不同应激的影响。PH酸性或氧化应激能转变拓扑异构酶到DNA破坏核酸酶，这引起基因组的不稳定性和细胞死亡。DNA破坏拓扑异构酶靶点药物(e.g. etoposide)也能将拓扑异构酶转变成核酸酶，并且该酶通常被诱捕作为中间物，共价地结合到剪切的DNA链的5+末端。该中间物再次导致基因组的不稳定性和细胞死亡，因此这些针对拓扑异构酶的因子是在癌症化学治疗药物之后高度寻找的(3)。Ca2+调节topoisomerase IIa蛋白Ser1106位点的磷酸化可调节该酶的活性和对靶药物的敏感性(4)。

1. Wang, J.C. (2002) Nat. Rev. Mol. Cell. Biol. 3, 430-440.
2. Pulleyblank, .E. (1997) Science 277, 648-649.
3. Li, T.K. and Liu, L.F. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 53-77.
4. Chikamori, K. et al. (2003) J. Biol. Chem. 278, 12696-12702.

原厂资料：

Specificity / Sensitivity

Topoisomerase IIalpha Antibody detects endogenous levels of total Topoisomerase IIalpha protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Topoisomerase IIalpha. Antibodies are purified by protein A and peptide affinity chromatography.

Background

DNA topoisomerase I and II are nuclear enzymes, and type II consists of two highly homologous isoforms: topoisomerase IIalpha and IIbeta. These enzymes regulate the topology of DNA, maintain genomic integrity and are essential for processes such as DNA replication, recombination, transcription and chromosome segregation by allowing DNA strands to pass through each other (1). Topoisomerase I nicks and rejoins one strand of the duplex DNA, but topoisomerase II transiently breaks and closes double-stranded DNA (2). Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g. etoposide) also convert topoisomerases to nucleases, and the enzyme is usually trapped as an intermediate, covalently bound to the 5+ end of the cleaved DNA strand(s). This intermediate leads again to genomic instability and cell death, and thus these agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs (3). Ca2+ regulated phosphorylation of topoisomerase IIa Ser1106 modulates the activity of this enzyme as well as its sensitivity to targeting drugs (4).

1. Wang, J.C. (2002) Nat. Rev. Mol. Cell. Biol. 3, 430-440.
2. Pulleyblank, .E. (1997) Science 277, 648-649.
3. Li, T.K. and Liu, L.F. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 53-77.
4. Chikamori, K. et al. (2003) J. Biol. Chem. 278, 12696-12702.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.