ORC1 (7A7) Rat mAb

• 产品编号：CST-4731S      品牌：CST       原厂货号：4731S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

 ORC1 (7A7) Rat mAb 能够识别内源性ORC1总蛋白。该抗体不与其他ORC亚基发生交叉反应。该单克隆抗体是由带有His6标签的人重组ORC1蛋白免疫动物生产的。起始识别复合物(ORC)是一种高度保守的异源六聚化蛋白复合体，它与DNA复制起始位点处或其附近的DNA相关。ORC的六个亚基是DNA复制起始必不可少的(1-3)，而且ORC可能参与应激响应过程中的基因表达(4)。ORC结合DNA后允许其它蛋白如cdc6和MCMs的有序结合，从而形成前复制复合体(Pre-RCs)。Pre-RCs形成于细胞周期的末期和G1早期之间并伴随DNA合成的发生而被失活，允许了DNA复制和细胞分化的协同调节(5)。ORC单个或多个亚基的修饰可能是其S期失活的主要原因，而细胞分化阶段ORC亚基的染色质结合行为仍处于研究之中(6-7)。

1. Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624-27630.
2. Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428-12435.
3. Gibson, D.G. et al. (2006) Genes Cells 11, 557-573.
4. Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763-776.
5. Rowles, A. and Blow, J.J. (1997) Curr. Opin. Genet. Dev. 7, 152-157.
6. DePamphilis, M.L. (2003) Gene 310, 1-15.
7. McNairn, A.J. et al. (2005) Exp. Cell. Res. 308, 345-356.

原厂资料：

Specificity / Sensitivity

ORC1 (7A7) Rat mAb recognizes endogenous levels of total ORC1 protein. The antibody does not cross-react with other ORC subunits. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a His6-tagged recombinant human ORC1 protein.

Background

The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7). 

1. Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624-27630.
2. Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428-12435.
3. Gibson, D.G. et al. (2006) Genes Cells 11, 557-573.
4. Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763-776.
5. Rowles, A. and Blow, J.J. (1997) Curr. Opin. Genet. Dev. 7, 152-157.
6. DePamphilis, M.L. (2003) Gene 310, 1-15.
7. McNairn, A.J. et al. (2005) Exp. Cell. Res. 308, 345-356.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.