TRAF2 (C192) Antibody

• 产品编号：CST-4724S      品牌：CST       原厂货号：4724S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TRAF2Antibody能够检测内源性水平人源TRAF2总蛋白。到生理条件下，未检测到与其它家族成员的交叉反应。该多克隆抗体是采用合成的人源TRAF2蛋白半胱氨酸192位周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。TRAFs (TNF受体相关因子)是一个多功能接头蛋白家族，能够结合表面受体并募集其它蛋白形成多蛋白信号复合物，促进细胞应答（1-3）。TRAF家族的成员有共同的羧基末端“TRAF结构域”，能够介导与其它蛋白的相互作用；许多成员同时包含氨基末端锌/环指基序。首先鉴定出的TRAF家族成员是TRAF1和TRAF2，发现它们能够与TNF受体2(TNFRII)的胞质结构域相互作用（4）。已知六个TRAF家族成员(TRAF1-6)作为细胞表面广泛受体的接头蛋白，参与调解细胞生存、增殖、分化和应激反应。最初研究证明，TRAF2与TNFRII发生相互作用，此后研究证明它与其它表面受体CD27、CD30、CD40、4-1BB、Ox40 HVEM/ ATAR和LMP-1相互作用（1-3）。TRAF2还与大量的细胞内蛋白质相互作用，包括TRADD、FADD、I-TRAF/TANK、TRIP、A20、c-IAP1和2、Casper、RIP和NIK，这有助于其对细胞存活的调节。显性抑制和基因敲除的研究表明，TRAF2在TNF介导的NF-kB的活化和MAPK/JNK激酶通路中起着重要的作用（5-7）。

1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
4. Rothe, M. et al. (1994) Cell 78, 681-692.
5. Yeh, W. C. et al. (1997) Immunity 7, 715-725.
6. Reinhard, C. et al. (1997) EMBO J. 16, 1080-1092.
7. Rothe, M. et al. (1995) Science 269, 1424-1427.

原厂资料：

Specificity / Sensitivity

TRAF2 Antibody detects endogenous levels of total human TRAF2 protein. No cross-reactivity was detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 192 of human TRAF2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. While TRAF2 was originally described through its interaction with TNFRII, it has since been shown to interact with other surface receptors including CD27, CD30, CD40, 4-1BB, Ox40, HVEM/ATAR and LMP-1 (1-3). TRAF2 also associates with a large number of intracellular proteins, including TRADD, FADD, I-TRAF/TANK, TRIP, A20, c-IAP1 and 2, Casper, RIP and NIK, which help to regulate cell survival. Dominant negative and knockout studies have shown that TRAF2 plays an important role in TNF-mediated activation of NF-kB and the MAPK/JNK kinase pathway (5-7).

1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
4. Rothe, M. et al. (1994) Cell 78, 681-692.
5. Yeh, W. C. et al. (1997) Immunity 7, 715-725.
6. Reinhard, C. et al. (1997) EMBO J. 16, 1080-1092.
7. Rothe, M. et al. (1995) Science 269, 1424-1427.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody