Snail (SN9H2) Rat mAb

• 产品编号：CST-4719S      品牌：CST       原厂货号：4719S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Snail (SN9H2)Rat mAb 大鼠单抗识别内源性的总Snail 蛋白。单克隆抗体由人源Snail重组蛋白免疫动物产生。Snail 是锌指转录因子，能抑制E- cadherin转录，E- cadherin下调在胚胎发育中与上皮间质转换（EMT）有关，这与肿瘤细胞的侵袭过程有关（1-3）,事实上，E- cadherin表达与一些肿瘤侵袭表型正相关，在上皮癌细胞系的Snail和E- cadherin 在mRNA水平也存在负相关关系（4,5）。另外，Snail参与阻断细胞周期，抑制细胞死亡（6）。由GSK-3和 PAK1磷酸化的Snail也参与其蛋白稳定性，细胞定位和其他功能（7-10） .

1. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-3161.
2. Cano, A. et al. (2000) Nat. Cell Biol. 2, 76-83.
3. Batlle, E. et al. (2000) Nat. Cell Biol. 2, 84-89.
4. Yokoyama, K. et al. (2003) Int. J. Oncol. 22, 891-898.
5. Yokoyama, K. et al. (2001) Oral Oncol. 37, 65-71.
6. Vega, S. et al. (2004) Genes Dev. 18, 1131-1143.
7. Zhou, B.P. et al. (2004) Nat. Cell Biol. 6, 931-940.
8. Bachelder, R.E. et al. (2005) J. Cell Biol. 168, 29-33.
9. Yook, J.I. et al. (2005) J. Biol. Chem. 280, 11740-11748.
10. Yang, Z. et al. (2005) Cancer Res. 65, 3179-3184.

原厂资料：

Specificity / Sensitivity

Snail (SN9H2) Rat mAb detects endogenous levels of total Snail.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human Snail protein.

Background

Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (1-3). Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines (4,5). In addition, Snail blocks the cell cycle and confers resistance to cell death (6). Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function (7-10).

1. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-3161.
2. Cano, A. et al. (2000) Nat. Cell Biol. 2, 76-83.
3. Batlle, E. et al. (2000) Nat. Cell Biol. 2, 84-89.
4. Yokoyama, K. et al. (2003) Int. J. Oncol. 22, 891-898.
5. Yokoyama, K. et al. (2001) Oral Oncol. 37, 65-71.
6. Vega, S. et al. (2004) Genes Dev. 18, 1131-1143.
7. Zhou, B.P. et al. (2004) Nat. Cell Biol. 6, 931-940.
8. Bachelder, R.E. et al. (2005) J. Cell Biol. 168, 29-33.
9. Yook, J.I. et al. (2005) J. Biol. Chem. 280, 11740-11748.
10. Yang, Z. et al. (2005) Cancer Res. 65, 3179-3184.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody