Integrin β5 Antibody

• 产品编号：CST-4708S      品牌：CST       原厂货号：4708S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

整合素β5抗体检测内源性总的Integrin β5水平。多抗由合成的肽段免疫动物产生，该合成肽段对应人integrin β5蛋白的789位赖氨酸附近残基。抗体由蛋白A和肽段亲和层析技术纯化。整合素是α/β异源二聚的细胞表面受体，它在细胞粘附和迁移以及生长和存活中起关键作用（1,2）。整合素家族包含至少18个α和8个β亚单元，形成24个带有独特组织分布和重叠配基特异性的已知的整合素（3）。整合素不仅在胞外环境（由外而内信号）刺激下传递信号到细胞，也能感应胞内反应，从而改变它们和胞外环境的相互作用（由内而外信号）（1,2）。αVβ5整合素在各种组织和细胞类型中表达，包括内皮细胞，上皮细胞核成纤维细胞（4）。 它在基质粘附到VN, FN, SPARC和骨唾液蛋白（5）中起作用，并在神经胶质瘤侵袭和转移的癌细胞中起功能（6,7）。αVβ5整合素在生长因子诱导的肿瘤血管生成中起主要作用，在此αVβ5整合素和生长因子协同调节上皮细胞增殖和存活（8）。

1. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
2. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
4. Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111.
5. Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788.
6. Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166.
7. Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072.
8. Alghisi, G.C. and Rüegg, C. (2006) Endothelium 13, 113-135.

原厂资料：

Specificity / Sensitivity

Integrin β5 Antibody detects endogenous levels of total integrin β5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys789 of human integrin β5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). 
 The αVβ5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). αVβ5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the αVβ5 integrin and growth factors regulates endothelial cell proliferation and survival (8).

1. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
2. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
4. Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111.
5. Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788.
6. Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166.
7. Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072.
8. Alghisi, G.C. and Rüegg, C. (2006) Endothelium 13, 113-135.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.