Integrin β4 Antibody

• 产品编号：CST-4707P      品牌：Cell Signaling Technology       原厂货号：4707P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

整合素β4抗体检测内源性总的Integrin β4水平。多抗由合成的肽段免疫动物产生，该合成肽段对应人integrin β4蛋白的1812位丝氨酸附近残基。抗体由蛋白A和肽段亲和层析技术纯化。整合素是α/β异源二聚的细胞表面受体，它在细胞粘附和迁移以及生长和存活中起关键作用（1,2）。整合素家族包含至少18个α和8个β亚单元，形成24个带有独特组织分布和重叠配基特异性的已知的整合素（3）。整合素不仅在胞外环境（由外而内信号）刺激下传递信号到细胞，也能感应胞内反应，从而改变它们和胞外环境的相互作用（由内而外信号）（1,2）。整合素β4与整合素α6在细胞膜表面配对，形成整合素α6β4异源二聚体，这是一个重要的层粘连蛋白受体，对半桥粒形成和基本上皮细胞和基膜之间的稳定粘附的支持起关键作用（4,5）。整合素β4是一种重要的生长因子诱导信号通路组分，涉及肿瘤形成和侵袭细胞的生长（6,7）。

1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
4. Schaapveld, R.Q. et al. (1998) J Cell Biol 142, 271-84.
5. Litjens, S.H. et al. (2006) Trends Cell Biol 16, 376-83.
6. Guo, W. et al. (2006) Cell 126, 489-502.
7. Bertotti, A. et al. (2006) J Cell Biol 175, 993-1003.

原厂资料：

Specificity / Sensitivity

Integrin β4 Antibody detects endogenous levels of total integrin β4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1812 of human integrin β4 protein. Antibodies are purified peptide affinity chromatography.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). 
 Integrin β4 pairs with integrin α6 on the cell surface membrane to form the integrin α6β4 heterodimer, an important laminin receptor that is essential for hemidesmosome formation and the support of stable adhesions between basal epithelial cells and the basement membrane (4,5). Integrin β4 is an important component in several growth factor induced signaling pathways that are involved in tumorigenesis and invasive cell growth (6,7).

1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
4. Schaapveld, R.Q. et al. (1998) J Cell Biol 142, 271-84.
5. Litjens, S.H. et al. (2006) Trends Cell Biol 16, 376-83.
6. Guo, W. et al. (2006) Cell 126, 489-502.
7. Bertotti, A. et al. (2006) J Cell Biol 175, 993-1003.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.