Integrin α5 Antibody

• 产品编号：CST-4705P      品牌：Cell Signaling Technology       原厂货号：4705P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

整合素α5抗体可以识别内源性总的整合素α5蛋白。合成对应人整合素α5第458位丝氨酸及其临近氨基酸残基序列的多肽免疫动物获得多克隆抗体。抗体经过蛋白A和肽亲和层析纯化获得。整合素是α/β异源二聚的细胞表面受体，它在细胞粘附和迁移以及生长和存活中起关键作用（1,2）。整合素家族包含至少18个α和8个β亚单元，形成24个带有独特组织分布和重叠配基特异性的已知的整合素（3）。整合素不仅在胞外环境（由外而内信号）刺激下传递信号到细胞，也能感应胞内反应，从而改变它们和胞外环境的相互作用（由内而外信号）（1,2）。整合素α5/β1涉及多种生物学过程包括胚胎发育，血管生成和肿瘤转移（4,5）。通过与它的纤连蛋白配体结合，α5/β1能够转导信号以调控细胞黏附，迁移，细胞基质聚合以及细胞骨架的重塑（6）。

1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
4. Kabir-Salmani, M. et al. (2004) Mol Hum Reprod 10, 91-7.
5. Kim, S. et al. (2000) J Biol Chem 275, 33920-8.
6. Miyamoto, S. et al. (1995) J Cell Biol 131, 791-805.

原厂资料：

Specificity / Sensitivity

Integrin α5 Antibody detects endogenous levels of total integrin α5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser458 of human integrin α5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). 
 Integrin α5/β1 is involved in multiple biological processes including embryonic development, angiogenesis and tumor metastasis (4,5). By interaction with its fibronectin ligand, α5/β1 transduces signals that regulate cell adhesion, migration, matrix assembly and cytoskeletal organization (6).

1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
4. Kabir-Salmani, M. et al. (2004) Mol Hum Reprod 10, 91-7.
5. Kim, S. et al. (2000) J Biol Chem 275, 33920-8.
6. Miyamoto, S. et al. (1995) J Cell Biol 131, 791-805.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.