SFRP1 Antibody

• 产品编号：CST-4690S      品牌：CST       原厂货号：4690S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SFRP1抗体识别内源性的总SFRP1蛋白。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源SFRP1蛋白的Gly48氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化。分泌型卷曲相关蛋白(SFRPs)显示与G蛋白偶联受体Frizzled的胞外富含半胱氨酸的Wnt结合结构域具有同源性和结构相似性[1,2]。目前为止，五种独立的SFRPs (SFRP1 至 5)已经在哺乳细胞中找到。这些分泌型蛋白通过直接抑制Wnt蛋白或结合Frizzled来阻止Wnt蛋白和其他受体相互作用的方式来抑制Wnt信号通路[3]。各种SFRPs差异性地结合和调节Wnt蛋白；这些蛋白显示独特的表达方式并对发育调节起重要作用[4-7]。SFRP蛋白显示肿瘤抑制因子功能，其表达和功能缺失与多种侵袭性癌正相关。SFRP1基因缺失和其启动子高度甲基化导致的基因沉默已在多种癌症中被报道。SFRP1和其他Wnt信号通路蛋白不正常表达与色素性视网膜炎有关[参见参考文献8]。

1. Dann, C.E. et al. (2001) Nature 412, 86-90.
2. Kawano, Y. and Kypta, R. (2003) J Cell Sci 116, 2627-34.
3. Bafico, A. et al. (1999) J Biol Chem 274, 16180-7.
4. Galli, L.M. et al. (2006) Dev Dyn 235, 681-90.
5. Wawrzak, D. et al. (2007) Biochem Biophys Res Commun 357, 1119-23.
6. Hu, E. et al. (1998) Biochem Biophys Res Commun 247, 287-93.
7. Mao, C. et al. (2000) Arterioscler Thromb Vasc Biol 20, 43-51.
8. Bovolenta, P. et al. (2008) J Cell Sci 121, 737-46.

原厂资料：

Specificity / Sensitivity

SFRP1 Antibody detects endogenous levels of total SFRP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly48 of human SFRP1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Secreted Frizzled-related proteins (SFRPs) display homology and structural similarity to the extracellular cysteine-rich Wnt-binding domain of the G protein-coupled receptor Frizzled (1,2). To date, five distinct SFRPs (SFRP1 to 5) have been found in mammalian cells. These secreted proteins typically act as antagonists to Wnt signaling by directly binding and inhibiting Wnt proteins, or by binding Frizzled to block Wnt protein interaction with the receptor (3). The various SFRPs bind and regulate Wnt proteins differentially; these proteins also display distinct expression patterns as they play important roles in regulating development (4-7). SFRP proteins appear to act as tumor suppressors, with loss of expression or function correlating with many invasive forms of cancer. Deletion of the corresponding SFRP1 gene and promoter hypermethylation leading to gene silencing has been reported in a number of cancers. Abnormal expression of SRFP1 and other Wnt signaling proteins is associated with some cases of retinitis pigmentosa (reviewed in 8).

1. Dann, C.E. et al. (2001) Nature 412, 86-90.
2. Kawano, Y. and Kypta, R. (2003) J Cell Sci 116, 2627-34.
3. Bafico, A. et al. (1999) J Biol Chem 274, 16180-7.
4. Galli, L.M. et al. (2006) Dev Dyn 235, 681-90.
5. Wawrzak, D. et al. (2007) Biochem Biophys Res Commun 357, 1119-23.
6. Hu, E. et al. (1998) Biochem Biophys Res Commun 247, 287-93.
7. Mao, C. et al. (2000) Arterioscler Thromb Vasc Biol 20, 43-51.
8. Bovolenta, P. et al. (2008) J Cell Sci 121, 737-46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.