DKK1 Antibody

• 产品编号：CST-4687S      品牌：CST       原厂货号：4687S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DKK1抗体识别转染水平的人源DKK1蛋白，此抗体与DKK2没有交叉反应。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源DKK1蛋白Gln75 氨基酸残基序列一致。抗体由肽段亲和层析技术纯化。Dickkopf (DKK)家族蛋白由四个成员DKK1, DKK2, DKK3 和DKK4，通过抑制Wnt共受体LRP5和LRP6起着分泌性Wnt拮抗剂的作用[1,2]。DKK成员含有两个半胱氨酸富集结构域，在那里存在十个非常保守的胱氨酸[3]。在动物发育过程中，这些蛋白在时间与空间上都存在表达调控[4]。DKK成员也和跨膜蛋白Kremen1 和 2有高亲和结合性，而Kremen1 和 2也调控Wnt信号通路[5,6]。DKK1最开始被确定为蟾蜍胚胎头部形成诱导因子[7]。对骨骼质量调节有重要作用[8-10]。已发现DKK1在肺癌、食管癌和荷尔蒙耐受乳腺癌中表达上调[11,12]。但DKK1表达在恶性黑素瘤好大肠癌中降低[13,14]。

1. Mao, B. et al. (2001) Nature 411, 321-5.
2. Niehrs, C. (2006) Oncogene 25, 7469-81.
3. Krupnik, V.E. et al. (1999) Gene 238, 301-13.
4. Monaghan, A.P. et al. (1999) Mech Dev 87, 45-56.
5. Mao, B. et al. (2002) Nature 417, 664-7.
6. Davidson, G. et al. (2002) Development 129, 5587-96.
7. Glinka, A. et al. (1998) Nature 391, 357-62.
8. Baron, R. and Rawadi, G. (2007) Curr Osteoporos Rep 5, 73-80.
9. MacDonald, B.T. et al. (2007) Bone 41, 331-9.
10. Diarra, D. et al. (2007) Nat Med 13, 156-63.
11. Forget, M.A. et al. (2007) Br J Cancer 96, 646-53.
12. Yamabuki, T. et al. (2007) Cancer Res 67, 2517-25.
13. Kuphal, S. et al. (2006) Oncogene 25, 5027-36.
14. Aguilera, O. et al. (2006) Oncogene 25, 4116-21.

原厂资料：

Specificity / Sensitivity

DKK1 Antibody detects transfected levels of DKK1 protein. This antibody does not cross-react with DKK2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln75 of human DKK1. Antibodies are purified by peptide affinity chromatography.

Background

Dickkopf (DKK) family proteins consist of four members DKK1, DKK2, DKK3 and DKK4 that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6).DKK1 was initially identified as an inducer of head formation in Xenopus embryos (7) and plays an important role in the regulation of bone mass (8-10). Increased levels of DKK1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers (11,12), while DKK1 expression is decreased in malignant melanoma and colorectal cancers (13,14).

1. Mao, B. et al. (2001) Nature 411, 321-5.
2. Niehrs, C. (2006) Oncogene 25, 7469-81.
3. Krupnik, V.E. et al. (1999) Gene 238, 301-13.
4. Monaghan, A.P. et al. (1999) Mech Dev 87, 45-56.
5. Mao, B. et al. (2002) Nature 417, 664-7.
6. Davidson, G. et al. (2002) Development 129, 5587-96.
7. Glinka, A. et al. (1998) Nature 391, 357-62.
8. Baron, R. and Rawadi, G. (2007) Curr Osteoporos Rep 5, 73-80.
9. MacDonald, B.T. et al. (2007) Bone 41, 331-9.
10. Diarra, D. et al. (2007) Nat Med 13, 156-63.
11. Forget, M.A. et al. (2007) Br J Cancer 96, 646-53.
12. Yamabuki, T. et al. (2007) Cancer Res 67, 2517-25.
13. Kuphal, S. et al. (2006) Oncogene 25, 5027-36.
14. Aguilera, O. et al. (2006) Oncogene 25, 4116-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.