Chronophin/PDXP (C85E3) Rabbit mAb

• 产品编号：CST-4686S      品牌：CST       原厂货号：4686S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Chronophin/PDXP (C85E3) Rabbit mAb检测内源性chronophin/PDXP总蛋白。使用重组的小鼠MBP-chronophin蛋白免疫动物从而制备出单克隆抗体。Cofilin蛋白是一个保守的剪切类肌动蛋白结合蛋白（actin-severing protein），对于依赖actin动力的生理过程需要Cofilin蛋白，包括胞质分裂和细胞迁移（1）。actin动力的调节需要在cofilin蛋白的磷酸化形式和非磷酸化形式之间控制循环 (2)。通过 LIMK或TESK激酶使cofilin蛋白保守的氨基末端 Ser3位点磷酸化，cofilin蛋白剪切活性被抑制(3,4)。Slingshot (SSH)磷脂酶有三个哺乳类亚型被鉴定，其在体内可以使cofilin蛋白去磷酸化(5)。Chronophin (CIN, PDXP)是一个haloacid dehalogenase磷酸酶，其也可使cofilin蛋白去磷酸化。通过野生型或磷酸酶失活的突变CIN蛋白的过表达，CIN活性的改变干扰actin动力、细胞形态和细胞分裂 (6)。

1. Carlier, M.F. et al. (1999) J. Biol. Chem. 274, 33827-33830.
2. Huang, T.Y. et al. (2006) Curr. Opin. Cell Biol. 18, 26-31.
3. Arber, S. et al. (1998) Nature 393, 805-809.
4. Toshima, J. et al. (2001) J. Biol. Chem. 276, 31449-31458.
5. Niwa, R. et al. (2002) Cell 108, 233-246.
6. Gohla, A. et al. (2005) Nat. Cell Biol. 7, 21-29.

原厂资料：

Specificity / Sensitivity

Chronophin/PDXP (C85E3) Rabbit mAb detects endogenous levels of total chronophin/PDXP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant mouse MBP-chronophin. 

Background

Cofilin is a conserved actin-severing protein required for processes that rely on actin dynamics, including cytokinesis and cell motility (reviewed in 1). Regulation of actin dynamics requires the controlled cycling between the phosphorylated and unphosphorylated forms of cofilin (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at the conserved amino-terminal Ser3 of cofilin (3,4). Slingshot (SSH) phosphatase, for which there have been three mammalian isoforms identified, dephosphorylates cofilin in vivo (5). Chronophin (CIN, PDXP) is a haloacid dehalogenase phosphatase that also dephosphorylates cofilin. Alteration of CIN activity through overexpression of either the wildtype or phosphatase-inactive mutant CIN interferes with actin dynamics, cell morphology and cytokinesis (6). 

1. Carlier, M.F. et al. (1999) J. Biol. Chem. 274, 33827-33830.
2. Huang, T.Y. et al. (2006) Curr. Opin. Cell Biol. 18, 26-31.
3. Arber, S. et al. (1998) Nature 393, 805-809.
4. Toshima, J. et al. (2001) J. Biol. Chem. 276, 31449-31458.
5. Niwa, R. et al. (2002) Cell 108, 233-246.
6. Gohla, A. et al. (2005) Nat. Cell Biol. 7, 21-29.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.