DKK2 (G44) Antibody

• 产品编号：CST-4683S      品牌：CST       原厂货号：4683S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DKK2 (G44)抗体识别转染水平的人源DKK2蛋白，此抗体与DKK1没有交叉反应。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源DKK2蛋白Gly44氨基酸残基序列一致。抗体由肽段亲和层析技术纯化。Dickkopf (DKK)家族蛋白由四个成员DKK1, DKK2, DKK3 和DKK4，通过抑制Wnt共受体LRP5和LRP6起着分泌性Wnt拮抗剂的作用[1,2]。DKK成员含有两个半胱氨酸富集结构域，在那里存在十个非常保守的胱氨酸[3]。在动物发育过程中，这些蛋白在时间与空间上都存在表达调控[4]。DKK成员也和跨膜蛋白Kremen1 和 2有高亲和结合性，而Kremen1 和 2也调控Wnt信号通路[5,6]。DKK2对成骨细胞终端分化以及矿化基质的形成起作用，是来自角膜上皮祖细胞的无角化的复层上皮的分化所必需的[7,8]。

1. Mao, B. et al. (2001) Nature 411, 321-5.
2. Niehrs, C. (2006) Oncogene 25, 7469-81.
3. Krupnik, V.E. et al. (1999) Gene 238, 301-13.
4. Monaghan, A.P. et al. (1999) Mech Dev 87, 45-56.
5. Mao, B. et al. (2002) Nature 417, 664-7.
6. Davidson, G. et al. (2002) Development 129, 5587-96.
7. Li, X. et al. (2005) Nat Genet 37, 945-52.
8. Mukhopadhyay, M. et al. (2006) Development 133, 2149-54.

原厂资料：

Specificity / Sensitivity

DKK2 (G44) Antibody detects transfected levels of human DKK2 protein. This antibody does not cross-react with DKK1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly44 of human DKK2. Antibodies are purified by peptide affinity chromatography.

Background

Dickkopf (DKK) family proteins consist of four members DKK1, DKK2, DKK3 and DKK4 that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6).DKK2 plays a role in osteoblast terminal differentiation and formation of mineralized matrix and is required for differentiation of non-keratinizing stratified epithelium from the corneal epithelial progenitors (7,8).

1. Mao, B. et al. (2001) Nature 411, 321-5.
2. Niehrs, C. (2006) Oncogene 25, 7469-81.
3. Krupnik, V.E. et al. (1999) Gene 238, 301-13.
4. Monaghan, A.P. et al. (1999) Mech Dev 87, 45-56.
5. Mao, B. et al. (2002) Nature 417, 664-7.
6. Davidson, G. et al. (2002) Development 129, 5587-96.
7. Li, X. et al. (2005) Nat Genet 37, 945-52.
8. Mukhopadhyay, M. et al. (2006) Development 133, 2149-54.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody