Phospho-p73 (Tyr99) Antibody

• 产品编号：CST-4665S      品牌：CST       原厂货号：4665S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-p73 (Tyr99) Antibody 能够检测酪氨酸（99位）磷酸化的内源性p73蛋白。该抗体与酪氨酸（149位）磷酸化的p63蛋白发生交叉反应(见免疫沉淀图)。该多克隆抗体是由合成的人源的针对p73蛋白酪氨酸（99位）的多肽免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。

.p53家族成员，p73，以多种亚型/剪接变异体存在，它可以通过转录调节器的活性诱导细胞凋亡和细胞周期阻滞以应答DNA损伤（1-3）。 DNA损伤后，p73的Tyr99被c-Abl磷酸化，引起核基质迁移（4）。 DNA损伤诱导p73的Lys321乙酰化，该过程由乙酰转移酶p300介导，已报道它以增强包括P53AIP1（5）在内的基因转录。然而，另一份报告表明，p300不能在体乙酰化全长p73（6）。

While the sequences surrounding p73 Tyr99 and p63 Tyr149 are very similar, only p73 co-localizes with c-Abl following gamma irradiation, suggesting that p63 is not a c-Abl substrate (7).尽管p73蛋白酪氨酸（99位）与p63酪氨酸（149位）的序列非常相似，当gamma辐射后，仅p73与c-Ab1共定位，提示p63不是c-Ab1的底物。

1. Kaghad, M. et al. (1997) Cell 90, 809-19.
2. Jost, C.A. et al. (1997) Nature 389, 191-4.
3. De Laurenzi, V.D. et al. (1999) Cell Death Differ 6, 389-90.
4. Ben-Yehoyada, M. et al. (2003) J Biol Chem 278, 34475-82.
5. Costanzo, A. et al. (2002) Mol Cell 9, 175-86.
6. Zeng, X. et al. (2001) J Biol Chem 276, 48-52.
7. Hamer, G. et al. (2001) Oncogene 20, 4298-4304.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Phospho-p73 (Tyr99) Antibody detects endogenous levels of p73 only when phosphorylated at tyrosine 99. This antibody cross-reacts with p63 when phosphorylated at tyrosine 149 (see immunoprecipitation figure).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr99 of human p73. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full length p73 in vivo (6).While the sequences surrounding p73 Tyr99 and p63 Tyr149 are very similar, only p73 co-localizes with c-Abl following gamma irradiation, suggesting that p63 is not a c-Abl substrate (7).

1. Kaghad, M. et al. (1997) Cell 90, 809-19.
2. Jost, C.A. et al. (1997) Nature 389, 191-4.
3. De Laurenzi, V.D. et al. (1999) Cell Death Differ 6, 389-90.
4. Ben-Yehoyada, M. et al. (2003) J Biol Chem 278, 34475-82.
5. Costanzo, A. et al. (2002) Mol Cell 9, 175-86.
6. Zeng, X. et al. (2001) J Biol Chem 276, 48-52.
7. Hamer, G. et al. (2001) Oncogene 20, 4298-4304.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.