Phospho-Doublecortin (Ser297) Antibody

• 产品编号：CST-4605S      品牌：CST       原厂货号：4605S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Doublecortin (Ser297) Antibody 兔多抗识别内源性的Ser297磷酸化的doublecortin蛋白。该多克隆抗体由合成的人类doublecortin中Ser297位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。Doublecortin的突变可以导致无脑回畸形，一种伴有癫痫发作和智力迟滞的神经迁移障碍（1）。Doublecortin是一个微管相关的蛋白，可以稳定和束集微管蛋白。Doublecortin中的一个保守结构域介导其与微管的相互作用，有趣的是，大多数无义突变都发生在这个区域（2）。JNK、CDK5和PKA激酶可以磷酸化Doublecortin。JNK磷酸化Thr321、Thr331和Ser334；PKA磷酸化Ser47；CDK5磷酸化Ser297（3-5）。Ser297的磷酸化降低了Doublecortin与微管的结合。另外，Ser297突变可以导致迁移障碍（5）。

1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

Phospho-Doublecortin (Ser297) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser297.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser297 of doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).

1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.