Insulin Antibody

• 产品编号：CST-4590S      品牌：CST       原厂货号：4590S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Insulin Antibody用于检测内源性Insulin蛋白水平。多克隆抗体通过用对应于人源Insulin蛋白序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。血糖平衡的维持是由激素调节的重要的生理过程。饮食后血糖的升高会通过一种血糖感应的途径来刺激胰岛β细胞分泌胰岛素（1）。胰岛素由前体形式合成，随后加工成A肽和B肽，A肽和B肽由二硫键相连形成成熟的胰岛素，并分泌到血液中去，而前体胰岛素中部段被剪切成C肽，释放出来。胰岛素会刺激骨骼肌和脂肪细胞从血液中摄取血糖。胰岛素的缺乏会导致1型糖尿病（2）。

1. Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
2. Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.

原厂资料：

Specificity / Sensitivity

Insulin Antibody detects endogenous levels of total insulin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).

1. Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
2. Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.