GATA-1 (D24E4) XP? Rabbit mAb

• 产品编号：CST-4589S      品牌：CST       原厂货号：4589S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GATA-1 (D24E4) XP^® Rabbit mAb兔单抗识别内源性的GATA-1蛋白。单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源GATA-1蛋白的Ala184氨基酸残基序列一致。GATA蛋白含有一组含有保守的锌指DNA结合结构域的转录因子，能够直接和核心元件GATA核苷酸序列结合(1-3)。共有六种脊椎动物GATA蛋白，GATA-1到GATA-6(3)。GATA-1是GATA家族主要成员，为红细胞和巨核细胞发育所必需[4,5]。对应GATA-1的突变与多种人类疾病相关，包括在唐氏综合症儿童中的急性巨核细胞白血病(DS-AMKL), X连锁血小板减少症，灰色血小板综合征[6-9]。

1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
4. Pevny, L. et al. (1991) Nature 349, 257-60.
5. Fujiwara, Y. et al. (1996) Proc Natl Acad Sci USA 93, 12355-8.
6. Wechsler, J. et al. (2002) Nat Genet 32, 148-52.
7. Cantor, A.B. (2005) Int J Hematol 81, 378-84.
8. Mehaffey, M.G. et al. (2001) Blood 98, 2681-8.
9. Tubman, V.N. et al. (2007) Blood 109, 3297-9.

原厂资料：

Specificity / Sensitivity

GATA-1 (D24E4) XP^® Rabbit mAb detects endogenous levels of GATA-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala184 of human GATA-1.

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (4,5). Mutations in the corresponding GATA-1 gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (6-9).

1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
4. Pevny, L. et al. (1991) Nature 349, 257-60.
5. Fujiwara, Y. et al. (1996) Proc Natl Acad Sci USA 93, 12355-8.
6. Wechsler, J. et al. (2002) Nat Genet 32, 148-52.
7. Cantor, A.B. (2005) Int J Hematol 81, 378-84.
8. Mehaffey, M.G. et al. (2001) Blood 98, 2681-8.
9. Tubman, V.N. et al. (2007) Blood 109, 3297-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.