Lyn (5G2) Mouse mAb

• 产品编号：CST-4576S      品牌：CST       原厂货号：4576S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Lyn (5G2) Mouse mAb能够检测内源Lyn蛋白总体水平。此抗体可能与Src家族的其它成员交叉反应。此单克隆抗体是通过合成具有His标签的人Lyn融合蛋白来免疫动物而获得。Lyn作为 Src 家族的一员在造血细胞中高表达(1)。据报道两个酪氨酸残基对Src家族中的酪氨酸激酶调控发挥了及其重要的作用。催化区域 Tyr396位点的自磷酸化 (等同于 Src 的 Tyr416 位点 )与酶活性有很大的关联。 Csk-介导的C-末端Tyr507位点的磷酸化 (等同于 Src 的 Tyr527 位点)能使酶失活。 酪氨酸激酶的磷酸化和 Lyn的激活与细胞表面受体BCR和 CD40 有关(2-4)。用基因敲除老鼠研究表明Lyn缺失的影响是刺激B细胞而对BCR的刺激过敏反应(5-7), 这就表明Lyn在体内的作用主要是在B细胞应答的下游。Lyn也参与到控制特殊B细胞类群的迁移和发育(8)。

1. Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
2. Yamanashi, Y. et al. (1991) Science 251, 192-194.
3. Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7410-7414.
4. Ren, C.L. et al. (1994) J. Exp. Med. 179, 673-680.
5. Wang, J. et al. (1996) J. Exp. Med. 184, 831-838.
6. Chan, V.W. et al. (1997) Immunity 7, 69-81.
7. Hibbs, M. L. et al. (1995) Cell 83, 301-311.
8. Seo, S.J. et al. (2001) J. Immunol. 166, 3710-3723.

原厂资料：

Specificity / Sensitivity

Lyn (5G2) Mouse mAb detects endogenous levels of total Lyn protein. This antibody may cross-react with other Src-family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant His-tagged human Lyn protein.

Background

Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8).

1. Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
2. Yamanashi, Y. et al. (1991) Science 251, 192-194.
3. Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7410-7414.
4. Ren, C.L. et al. (1994) J. Exp. Med. 179, 673-680.
5. Wang, J. et al. (1996) J. Exp. Med. 184, 831-838.
6. Chan, V.W. et al. (1997) Immunity 7, 69-81.
7. Hibbs, M. L. et al. (1995) Cell 83, 301-311.
8. Seo, S.J. et al. (2001) J. Immunol. 166, 3710-3723.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody