Cool2/αPix (C23D2) Rabbit mAb

• 产品编号：CST-4573S      品牌：CST       原厂货号：4573S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cool2/αPix (C23D2) Rabbit mAb检测内源性Cool2/αPix总蛋白。通过人工合成人源Cool2/αPix蛋白羧基末端相应的多肽片段去免疫动物从而制备出单克隆抗体。Cool/Pix蛋白是由定位于粘着斑的鸟嘌呤核苷交换因子（guanine nucleotide exchange factors，GEFs）家族组成。该家族包含有两个亚型：cool2/αpix and cool1/βPix，后者有两个剪切变异体，其区别是在它们的羧基终端(1)。像其它GEFs，Cool2/αPix蛋白有一个DH (Dbl homology)功能域，其能允许小GTPases的结合和GDP/GTP的交换，和一个PH (Pleckstrin homology) 功能域(2)。在非特异性精神发育迟滞（ nonspecific mental retardation，MRX）中X-chromosomal突变的基因包含一个基因家族，包括编码Cool2/αPix的基因，被认为涉及精神发育迟滞(3,4)。Cool2/αPix蛋白与β-parvin/affixin蛋白相互作用，而 β-parvin/affixin蛋白涉及整合蛋白信号(5)，并且Cool2/αPix蛋白可能作用integrin-linked kinase (ILK)的下游去调节actin 蛋白的重组和细胞铺展(6)。当Cool2αPix蛋白以一个二聚体存在，它的功能作为一个Rac-specific GEF，然而单体存在的蛋白对于Rac和Cdc42蛋白作为一个GEF。在细胞外信号反应下，通过p21 activated kinase (PAK)Cool2/αPix蛋白二聚化的调节和它的特异性在某种程度上发生(7)。进一步，Cdc42蛋白的结合提高Cool2/αPix二聚体的Rac GEF 活性。活化的Rac轮流地抑制Cool2/αPix Rac GEF活性(8)。

1. Koh, C.G. et al. (2001) J Cell Sci 114, 4239-51.
2. Kutsche, K. and Gal, A. (2001) Cytogenet Cell Genet 95, 196-201.
3. Kutsche, K. et al. (2000) Nat Genet 26, 247-50.
4. Lower, K.M. and Gecz, J. (2001) Am J Med Genet 100, 43-8.
5. Rosenberger, G. et al. (2003) Hum Mol Genet 12, 155-67.
6. Filipenko, N.R. et al. (2005) Oncogene 24, 5837-49.
7. Feng, Q. et al. (2004) EMBO J 23, 3492-504.
8. Baird, D. et al. (2005) Curr Biol 15, 1-10.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Dog

Specificity / Sensitivity

Cool2/αPix (C23D2) Rabbit mAb detects endogenous levels of total Cool2/αPix protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Cool2/αPix. 

Background

Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/αPix and cool1/βPix, the latter having two splice variants that vary in their carboxy termini (1). Cool2/αPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain (2). 
 X-chromosomal genes mutated in nonspecific mental retardation (MRX) comprise a family of genes, including the gene encoding Cool2/αPix, thought to be involved in mental retardation (3,4). 
 Cool2/αPix interacts with β-parvin/affixin, a protein involved in integrin signaling (5), and may act downstream of integrin-linked kinase (ILK) to regulate actin reorganization and cell spreading (6). 
 When Cool2αPix exists as a dimer, it functions as a Rac-specific GEF, whereas the monomeric protein acts as a GEF for both Rac and Cdc42. Regulation of Cool2/αPix dimerization, and therefore its specificity, occurs at least in part through p21 activated kinase (PAK) in response to extracellular signaling (7). Further, binding of Cdc42 enhances the Rac GEF activity of the Cool2/αPix dimer. Activated Rac in turn inhibits Cool2/αPix Rac GEF activity (8). 

1. Koh, C.G. et al. (2001) J Cell Sci 114, 4239-51.
2. Kutsche, K. and Gal, A. (2001) Cytogenet Cell Genet 95, 196-201.
3. Kutsche, K. et al. (2000) Nat Genet 26, 247-50.
4. Lower, K.M. and Gecz, J. (2001) Am J Med Genet 100, 43-8.
5. Rosenberger, G. et al. (2003) Hum Mol Genet 12, 155-67.
6. Filipenko, N.R. et al. (2005) Oncogene 24, 5837-49.
7. Feng, Q. et al. (2004) EMBO J 23, 3492-504.
8. Baird, D. et al. (2005) Curr Biol 15, 1-10.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody