Phospho-Cortactin (Tyr421) Antibody

• 产品编号：CST-4569S      品牌：CST       原厂货号：4569S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Cortactin (Tyr421) Antibody仅检测Tyr421位点磷酸化的内源性cortactin蛋白。通过人工合成人源cortactin蛋白Tyr421位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。皮层蛋白(Cortactin)是一个皮层肌动蛋白结合蛋白。它的氨基端酸性结构域(amino terminal acidic domain, NTA)在 F-actin分支与Arp2/3和WASP复合物紧密联系。该蛋白的中心区域包含六个37氨基酸的重复序列，该序列在F-actin的结合和交联中发挥重要作用。该蛋白的羧基末端包含一个脯氨酸富足区域和SH3 功能域，后者与许多支架蛋白相互作用，包括CortBP1和Shank3蛋白(1,2)。Cortactin蛋白是涉及到在细胞移动中协调actin重组的信号活动。人源cortactin蛋白同系物EMS1蛋白在许多病人的不佳预后癌症中高表达(3)。Cortactin蛋白可能也在突触后致密（postsynaptic density ，PSD）的组织和通过联系支架蛋白到actin蛋白网络的紧密连接中起到重要作用(4)。Cortactin蛋白在酪氨酸残基421、466和482位点磷酸化。cortactin 蛋白的酪氨酸位点磷酸化调节细胞迁移(5)、 rac1介导actin动力(6)、钙粘蛋白依赖的粘附(7)、趋化因子的交换和趋化因子依赖的趋化作用(8)。

1. Du, Y. et al. (1998) Mol Cell Biol 18, 5838-51.
2. Naisbitt, S. et al. (1999) Neuron 23, 569-82.
3. Rodrigo, J.P. et al. (2000) Clin Cancer Res 6, 3177-82.
4. Weed, S.A. and Parsons, J.T. (2001) Oncogene 20, 6418-34.
5. Huang, C. et al. (1998) J Biol Chem 273, 25770-6.
6. Head, J.A. et al. (2003) Mol Biol Cell 14, 3216-29.
7. El Sayegh, T.Y. et al. (2004) J Cell Sci 117, 5117-31.
8. Luo, C. et al. (2006) J Biol Chem 281, 30081-93.

原厂资料：

Specificity / Sensitivity

Phospho-Cortactin (Tyr421) Antibody detects endogenous levels of cortactin protein only when phosphorylated at Tyr421.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr421 of human cortactin. Antibodies are purified using protein A and peptide affinity chromatography.

Background

Cortactin is a cortical actin binding protein. Its amino-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3 (1,2). Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis (3). Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network (4). 
 Cortactin is phosphorylated at tyrosine residues 421, 466, and 482. Tyrosine phosphorylation of cortactin regulates cell motility (5), rac1-mediated actin dynamics (6), cadherin-dependent adhesion (7), chemokine trafficking and chemokine-dependent chemotaxis (8).

1. Du, Y. et al. (1998) Mol Cell Biol 18, 5838-51.
2. Naisbitt, S. et al. (1999) Neuron 23, 569-82.
3. Rodrigo, J.P. et al. (2000) Clin Cancer Res 6, 3177-82.
4. Weed, S.A. and Parsons, J.T. (2001) Oncogene 20, 6418-34.
5. Huang, C. et al. (1998) J Biol Chem 273, 25770-6.
6. Head, J.A. et al. (2003) Mol Biol Cell 14, 3216-29.
7. El Sayegh, T.Y. et al. (2004) J Cell Sci 117, 5117-31.
8. Luo, C. et al. (2006) J Biol Chem 281, 30081-93.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.