Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb

• 产品编号：CST-4568S      品牌：CST       原厂货号：4568S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb兔单抗用于检测内源性在Tyr980位点磷酸化的IGF-I β蛋白水平。该抗体可与激活型的胰岛素受体和FLT3交互反应。单克隆抗体通过用对应于人源磷酸化IGF-I β蛋白Tyr980序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。一型胰岛素样生长因子受体(IGF-IR)是一种跨膜的酪氨酸激酶，在许多新生及成熟的组织细胞中都广泛表达(1-3)。IGF-I和IGF-II配体结合到受体上去，受体就发生自我磷酸化。在激酶结构域，有三个酪氨酸残基(Tyr1131, Tyr1135,Tyr1136)是最先发生自身磷酸化的位点(4)。这三个残基的磷酸化对激酶的激活是必需的(5,6)。胰岛素受体(IRs)跟胰岛素样生长因子受体有着相似的结构和功能，在它的激酶活化环上同样有三个酪氨酸簇(Tyr1146/1150/1151)。胰岛素受体酪氨酸残基的磷酸化是细胞接受胰岛素刺激后最先发生的生化反应(7)。自身磷酸化最先在Tyr1146和Tyr1150(或Tyr1151)发生，但激酶的激活需要三者同时磷酸化(8)。IGF-1R的Tyr980对于受体激酶的活性是很重要的。该残基位于受体的膜旁区，该酪氨酸残基的磷酸化为下游的接头蛋白等停靠蛋白提供了一个供停靠的结合位点（9）。

1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
2. Baserga, R. (2000) Oncogene 19, 5574-81.
3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.
9. Pautsch, A. et al. (2001) Structure 9, 955-965.

原厂资料：

Specificity / Sensitivity

Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb detects endogenous levels of IGF-I β receptor protein when phosphorylated at Tyr980. The antibody may cross-react with activated insulin receptors and FLT3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr980 of human IGF-I Receptor β.

Background

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).Tyr980 of IGF-IR appears to be important for receptor kinase activation. Located in the IGF-IR juxtamembrane region, phosphorylation of this tyrosine residue creates a docking site for the binding of downstream adaptor or docking proteins (9).

1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
2. Baserga, R. (2000) Oncogene 19, 5574-81.
3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.
9. Pautsch, A. et al. (2001) Structure 9, 955-965.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.