Phospho-TNK1 (Tyr277) Antibody

• 产品编号：CST-4553S      品牌：CST       原厂货号：4553S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-TNK1 (Tyr277) Antibody兔多抗能检测内源性Tyr277位点磷酸化的TNK1蛋白水平。该抗体可能与酪氨酸磷酸化的EGF受体发生交叉反应。该多克隆抗体通过使用与人源TNK1蛋白Tyr277位点周围序列相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。酪氨酸激酶非受体1(TNK1)与Ack1 (TNK2)非受体激酶相关，能够结合cdc42并抑制该细胞周期调节子的GTP酶活性 。TNK1广泛表达于胚胎发生组织和白血病细胞系中，但是在成人组织中的表达有所限制(1)。TNK1蛋白的大小大约是72 kDa，由N端激酶结构域、中心SH3结构域和一个富含脯氨酸的尾部区域组成(2)。尽管确切的发生机制目前尚不清楚，但是活化的TNK1蛋白可能会通过抑制TNF-α诱导的NF-κB转录激活而调节细胞死亡(3)。TNK1的Tyr277位点的磷酸化由Cell Signaling Technology (CST)发现，使用的技术是PhosphoScan®——这是我们用于发现磷酸化位点的LC-MS/MS平台(4)，同时也利用这项MS技术独立发表了文章(5)。在某些癌细胞系和肿瘤中观察到TNK1的Tyr277位点的磷酸化。在一些细胞中还观察到TNK1与C17orf61基因的融合导致截断了的TNK1激酶具有组成性活性(5)。更多信息详见PhosphoSitePlus™——CST的修饰位点信息库，网址是www.phosphosite.org.

1. Hoehn, G.T. et al. (1996) Oncogene 12, 903-13.
2. Felschow, D.M. et al. (2000) Biochem Biophys Res Commun 273, 294-301.
3. Azoitei, N. et al. (2007) Oncogene 26, 6536-45.
4. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
5. Gu, T.L. et al. (2010) Leukemia 24, 861-5.

原厂资料：

Specificity / Sensitivity

Phospho-TNK1 (Tyr277) Antibody detects endogenous levels of TNK1 protein only when phosphorylated at Tyr277. This antibody may cross-react with tyrosine phosphorylated EGF receptor.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence around Tyr277 of human TNK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tyrosine kinase non-receptor 1 (TNK1) is related to the Ack1 (TNK2) non-receptor kinase that binds cdc42 and inhibits GTPase activity of this cell cycle regulator. TNK1 is broadly expressed in embryogenic tissues and leukemia cell lines, but is restricted to select adult tissues (1). TNK1 is a putative 72 kDa protein comprised of an N-terminal kinase domain, a central SH3 domain and a proline-rich tail. Interaction with PLCγ in vitro indicates a possible role in phospholipid signal transduction pathways (2). Though the exact mechanism is currently unclear, active TNK1 may play a role in regulating cell death by preventing TNF-α-induced NF-κB transactivation (3).Phosphorylation of TNK1 on Tyr277 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (4) and also reported independently in another publication using MS technology (5). Phosphorylation of TNK1 at Tyr277 was observed in select carcinoma cell lines and in tumors. A constitutively active, truncated TNK1 kinase resulting from fusion between the TNK1 and C17orf61 genes is seen in some cells (5). For additional information visit PhosphoSitePlus™, CST's modification site knowledgebase, at www.phosphosite.org.

1. Hoehn, G.T. et al. (1996) Oncogene 12, 903-13.
2. Felschow, D.M. et al. (2000) Biochem Biophys Res Commun 273, 294-301.
3. Azoitei, N. et al. (2007) Oncogene 26, 6536-45.
4. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
5. Gu, T.L. et al. (2010) Leukemia 24, 861-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.