Phospho-MAP2 (Ser136) Antibody

• 产品编号：CST-4541S      品牌：CST       原厂货号：4541S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MAP2 (Ser136) Antibody 兔多抗识别内源性的Ser136磷酸化的MAP2蛋白。此抗体与磷酸化的tau蛋白有交叉反应。该多克隆抗体由合成的人类MAP2蛋白Ser136位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。微管相关蛋白2（MAP2）是一种神经元磷酸化蛋白，调节轴突和树突中微管的结构和稳定性，神经元的形态，细胞骨架动力学和细胞器的运输（1）。MAP2的多种亚型在神经元表达，其中包括超高分子量MAP2A和MAP2B（280和270 kDa），低分子量MAP2C和MAP2D（70和75 kDa）。微管相关蛋白的磷酸化调节其与细胞骨架的结合，并受到发育的调控。MAP2的丝氨酸136，苏氨酸1620和苏氨酸1623的磷酸化由GSK3和p44/42 MAP激酶完成（2,3）。GSK3对苏氨酸1620/1623的磷酸化抑制MAP2与微管蛋白结合，削弱微管稳定性（3）。

1. Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
2. Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
3. Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

Phospho-MAP2 (Ser136) Antibody detects endogenous levels of all isoforms of MAP2 only when phosphorylated at serine 136. This antibody does not cross-react with phosphorylated tau.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser136 of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Serine 136, threonine 1620 and threonine 1623 of MAP2 are phosphorylated by GSK3 and p44/42 MAP kinases (2,3). Phosphorylation at threonine 1620/1623 by GSK3 inhibits MAP2 association with microtubules and microtubule stability (3).

1. Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
2. Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
3. Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody