Phospho-TAK1 (Thr184/187) Antibody

• 产品编号：CST-4531S      品牌：CST       原厂货号：4531S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-TAK1 (Thr184/187) Antibody能检测内源性苏氨酸（184、187位点）共磷酸化的TAK1蛋白水平。在一些细胞裂解物中，该抗体能与苏氨酸（184位点）单磷酸化的TAK1蛋白发生微弱的交叉反应。该多克隆抗体通过使用人源TAK1蛋白苏氨酸（184、187位点）周围残基的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。TAK1是MAPKKK家族中的成员，由TGF-β、骨形态生成蛋白和包括IL-1在内的细胞因子所激活(1,2)。在体内，TAK1的活化需要它与TAK1结合蛋白1 (TAB1)的结合，这种结合能够使TAK1蛋白磷酸化(3,4)。另一个接头蛋白TAB2能够将TAK1与TRAF6连接起来，调节IL-1刺激引起的TAK1的活化(5)。一经激活，TAK1能够磷酸化MAPK激酶MKK4和MKK3/6，MKK4和MKK3/6再分别激活JNK和p38 MAPK。另外，TAK1能够激活NF-κB通路，这是通过与TRAF6的相互作用及对NF-κB诱导激酶(NIK)的磷酸化实现的 (2)。TAK1的活化需要它的活化环内许多位点的磷酸化。定位于TAK1活化环内苏氨酸187/184位点的突变将损害TAK1、TAB1的磷酸化，降低TAK1的激酶活性，暗示了这些位点的自磷酸化是TAK1活化所必需的(4)。

1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Chicken, Xenopus, Zebrafish, Bovine

Specificity / Sensitivity

Phospho-TAK1 (Thr184/187) Antibody detects endogenous levels of TAK1 only when phosphorylated at both threonine 184 and threonine 187. This antibody weakly cross-reacts with TAK1 singly phosphorylated at threonine 184.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a phosphopeptide corresponding to residues surrounding Thr184 and Thr187 of human TAK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).TAK1 activation requires multiple phosphorylations in its activation loop. Mutations of Thr187 and Thr184, residues located in the activation loop of TAK1, impairs phosphorylation of both TAK1 and TAB1 and reduces the kinase activity of TAK1, suggesting that autophosphorylation of these residues is necessary for TAK1 activation (4).

1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody