Pan-Keratin (C11) Mouse mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-4528S      品牌：CST       原厂货号：4528S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Pan-Keratin (C11) Mouse mAb (Alexa Fluor^® 647 偶联)识别内源性的keratin 4、5、6、8、10、13和18总蛋白，但是不能与其它keratin蛋白发生交叉反应。经A431细胞制备的一个细胞骨架蛋白，免疫动物从而获得单克隆抗体。该抗体以F/P 比值为2-6的最佳条件连接着Alexa Fluor^®647。Alexa Fluor^® 647染料最大限度的激发红光(e.g. 633 nm He-Ne laser)。连接着Alexa Fluor^® 647 dye的抗体能产生峰值为665 nm的远红外荧光发射。Cell Signaling Technology抗体连接着Alexa Fluor^® 647 荧光染料，并且直接用流式细胞仪和免疫荧光分析方法内部检测人类细胞。非连接的抗体#4545可与人源、大鼠和猴keratin 4、5、6、8、10、13和18蛋白发生反应。CST公司期望Pan-Keratin (C11) Mouse mAb (Alexa Fluor^® 647 偶联)也将识别这些物种的同样的keratin蛋白。角蛋白（细胞角蛋白）是中间丝蛋白主要在上皮细胞中表达。角蛋白的异二聚体由一个酸性角蛋白（或type I角蛋白，角蛋白9-23或）和一个碱性角蛋白（type II角蛋白，角蛋白1-8）聚合形成中间丝（1.2）。角蛋白的亚型在组织-和分化的特异性表达，这使他们可做为有用的生物标志（1）。研究显示角蛋白基因突变与肌肤失调，肝脏和胰腺疾病，以及肠道炎性疾病相关（3-6）。

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

原厂资料：

Specificity / Sensitivity

Pan-Keratin (C11) Mouse mAb (Alexa Fluor^® 647 Conjugate) detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A431 cells. This antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The unconjugated antibody #4545 reacts with keratins 4, 5, 6, 8, 10, 13 and 18 from human, rat and monkey. CST expects that Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) will also recognize the same keratins in these species.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.