Cool1/βPix Antibody

• 产品编号：CST-4515S      品牌：CST       原厂货号：4515S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

通过人工合成人源cool1/βPix蛋白中间区域相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Cool/Pix蛋白是由定位于粘着斑的鸟嘌呤核苷交换因子（guanine nucleotide exchange factors，GEFs）家族组成。该家族包含有两个亚型：cool2/αpix and cool1/βPix，后者有两个剪切变异体，其区别是在它们的羧基终端(1)。像其它GEFs，Cool1/βPix蛋白有一个DH (Dbl homology)功能域，其能允许小GTPases的结合和GDP/GTP的交换，和一个PH (Pleckstrin homology) 功能域，其在亚细胞的定位中有重要作用。Cool1/βPix蛋白也有一个SH3功能域，它结合cdc42和Rac激酶的下游效应因子PAK激酶(3,4)。通过MAPK信号的下游 PAK2蛋白使cool1/βPix蛋白的磷酸化改变包括 PAK2和cool-1/βPix的复合物的定位，在生长因子作用下调节生长锥（growth cone）的形成(4)。生长因子通过cool1/βPix 蛋白诱导Rac1蛋白的激活之后被显示发现在独立的亚细胞定位(5)。肾小球系膜细胞的Endothelin-1刺激蛋白激酶A(PKA)通路，导致cool-1/βPix蛋白的定位和cdc42蛋白的激活(6)。

1. Koh, C.G. et al. (2001) J. Cell Sci. 114, 4239-4251.
2. Manser, E. et al. (1998) Mol. Cell 1, 183-192.
3. Bagrodia, S. and Cerione, R.A. (1999) Trends Cell Biol. 9, 350-355.
4. Shin, E.Y. et al. (2002) J. Biol. Chem. 277, 44417-44430.
5. Shin, E.Y. et al. (2004) J. Biol. Chem. 279, 1994-2004.
6. Chahdi, A. et al. (2005) J. Biol. Chem. 280, 578-584.

原厂资料：

Specificity / Sensitivity

Cool1/βPix Antibody detects endogenous levels of total cool1/βPix protein. The antibody does not cross-react with cool2/αPix. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the center of human cool1/βPix. Antibodies are purified by peptide affinity chromatography.

Background

Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/αpix and cool1/βPix, the latter having two splice variants that vary in their carboxy termini (1). Cool1/βPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain, which is important in regulating subcellular localization. Cool1/βPix also has an SH3 domain, which binds to the PAK kinase, a downstream effector of cdc42 and Rac (3,4). Phosphorylation of cool1/βPix by PAK2 downstream of MAPK signaling alters the localization of a complex containing PAK2 and cool-1/βPix, regulating formation of growth cones in response to growth factors (4). Growth factor induced activation of Rac1 via cool1/βPix was later shown to occur independently of subcellular localization (5). Endothelin-1 stimulation of mesangial cells stimulates the protein kinase A (PKA) pathway, resulting in translocation of cool-1/βPix and activation of cdc42 (6). 

1. Koh, C.G. et al. (2001) J. Cell Sci. 114, 4239-4251.
2. Manser, E. et al. (1998) Mol. Cell 1, 183-192.
3. Bagrodia, S. and Cerione, R.A. (1999) Trends Cell Biol. 9, 350-355.
4. Shin, E.Y. et al. (2002) J. Biol. Chem. 277, 44417-44430.
5. Shin, E.Y. et al. (2004) J. Biol. Chem. 279, 1994-2004.
6. Chahdi, A. et al. (2005) J. Biol. Chem. 280, 578-584.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%sodium
azide. Store at –20°C. Do not aliquot the antibody.