Phospho-HS1 (Tyr397) Antibody

• 产品编号：CST-4507S      品牌：CST       原厂货号：4507S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-HS1 (Tyr397) 抗体只能检测内源的在Tyr397位点磷酸化的HS1蛋白。此多克隆抗体是通过合成人源对应的HS1 Tyr397位点周围的磷肽来免疫动物而获得。抗体是通过多肽亲和层析纯化。HS1 (HCLS1, LckBP1, p75)是蛋白激酶的底物，它只在具有造血功能的组织和细胞中表达(1,2)。HS1 包含了四个皮肌动蛋白重复序列和一个SH3 域(2)。在受到免疫受体的激活后此胞内蛋白被磷酸化,这就促进了HS1被招募到免疫突触(3-5)。磷酸化HS1是调控肌动蛋白动力不可缺少的，同时也为其他的信号分子如Vav1和 PLCγ1 提供了结合位点(6)。HS1也在血小板激活中发挥了重要的作用(7)。在免疫受体刺激和凝血酶介导的血小板刺激下HS1能够快速的被Syk和/或 Lyn激酶在Tyr397位点磷酸化. 此磷酸化是细胞骨架重排和信号复合体形成过程中重要的一步(6-10)。

1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.
8. Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
9. Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
10. Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.

原厂资料：

Specificity / Sensitivity

Phospho-HS1 (Tyr397) Antibody detects the endogenous level of HS1 protein when phosphorylated at Tyr397.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human HS1. Antibodies are purified using peptide affinity chromatography.

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).Tyr397 of HS1 protein is rapidly phosphorylated by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).

1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.
8. Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
9. Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
10. Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.