IRS-2 Antibody

• 产品编号：CST-4502S      品牌：CST       原厂货号：4502S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRS-2 Antibody用于检测内源性IRS-2蛋白水平，该抗体不与IRS-1蛋白交互反应。多克隆抗体通过用对应于人源IRS-2蛋白序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。胰岛素受体底物2(IRS-2)是胰岛素受体激酶的主要底物之一(1)。在脊椎动物中， IRS-2主要作为一个支架蛋白，用于协调Insulin/IGF信号级联反应中的各个分支通路(2)。IRS-2对于正常的营养稳态是必需的，因为它不仅介导外周组织中的胰岛素作用，还调节着IGF-1对于B细胞生长的作用。IRS-2缺失的小鼠不能维持足够量的胰岛素代偿性分泌，并且容易患1型糖尿病(3)。

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
3. Withers, D.J. et al. (1998) Nature 391, 900-904.

原厂资料：

Specificity / Sensitivity

IRS-2 Antibody detects endogenous levels of total IRS-2 protein. It does not cross-react with IRS-1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human IRS-2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
3. Withers, D.J. et al. (1998) Nature 391, 900-904.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.