Phospho-Tpl2 (Ser400) Antibody

• 产品编号：CST-4491S      品牌：CST       原厂货号：4491S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tpl2 (Ser400)兔多抗能检测转染水平的丝氨酸（400位点）磷酸化的Tpl2蛋白。该抗体不与其他磷酸化的MAP激酶发生交叉反应。该多克隆抗体是通过用与人、小鼠、大鼠Tpl2蛋白丝氨酸（400位点）周围残基相一致的磷酸化合成肽段混合物免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。Tpl2（肿瘤发展位点2）也被称为COT（大阪甲状腺癌）是一种丝氨酸/苏氨酸激酶，主要表达于造血组织、肺和肝脏中(1)。Tpl2的过度表达会通过MEK1和SEK1以及MKK6 和MEK5增强MAP激酶途径(2,3)。Tpl2也能通过NF-κB诱导激酶（NIK）或通过诱导NF-κB前体p105 NF-κB1的磷酸化和降解，参与NF-κB的活化(4,5)。Tpl2蛋白丝氨酸（400位点）被一个依赖Akt的方式磷酸化，该磷酸化是Tpl2诱导的NF-κB依赖型转录所必需的(6)。Tpl2蛋白也能通过促进一种蛋白复合体Apaf1 (凋亡蛋白酶活化因子 1)、caspase-9、 Tpl2、转接蛋白 Tvl1 和酶原-3的聚集而活化caspase-3(7)。

1. Patriotis, C. et al. (1993) Proc Natl Acad Sci U S A 90, 2251-5.
2. Salmeron, A. et al. (1996) EMBO J 15, 817-26.
3. Chiariello, M. et al. (2000) Mol Cell Biol 20, 1747-58.
4. Lin, X. et al. (1999) Immunity 10, 271-80.
5. Belich, M.P. et al. (1999) Nature 397, 363-8.
6. Kane, L.P. et al. (2002) Mol Cell Biol 22, 5962-74.
7. Patriotis, C. et al. (2001) J Cell Physiol 187, 176-87.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse

Specificity / Sensitivity

Phospho-Tpl2 (Ser400) Antibody detects transfected Tpl2 only when phosphorylated at Ser400. This antibody does not cross-react with other phosphorylated MAP kinase kinase kinases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a mixture synthetic phosphorylated peptides corresponding to residues surrounding Ser400 of human, mouse and rat Tpl2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tpl2 (tumor progression locus 2), also known as COT (cancer osaka thyroid), is a serine/threonine kinase expressed primarily in hematopoietic tissues, lung and liver (1). Over-expression of Tpl2 potentiates MAP kinase pathways through MEK1 and SEK1, as well as through MKK6 and MEK5 (2,3). Tpl2 is also engaged in NF-κB activation through NF-κB inducing kinase (NIK), or by inducing phosphorylation and degradation of the NF-κB precusor, p105 NF-κB1 (4,5). Ser400 of Tpl2 is phosphorylated in an Akt-dependent manner. This phosphorylation is required for Tpl2-induced NF-κB-dependent transcription (6). Tpl2 also activates caspase-3 by promoting the assembly of a protein complex of Apaf1 (apoptotic protease-activating factor 1), caspase-9, Tpl2, adaptor protein Tvl1 and procaspase-3 (7).

1. Patriotis, C. et al. (1993) Proc Natl Acad Sci U S A 90, 2251-5.
2. Salmeron, A. et al. (1996) EMBO J 15, 817-26.
3. Chiariello, M. et al. (2000) Mol Cell Biol 20, 1747-58.
4. Lin, X. et al. (1999) Immunity 10, 271-80.
5. Belich, M.P. et al. (1999) Nature 397, 363-8.
6. Kane, L.P. et al. (2002) Mol Cell Biol 22, 5962-74.
7. Patriotis, C. et al. (2001) J Cell Physiol 187, 176-87.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.