PTPmu (BK2) Mouse mAb

• 产品编号：CST-4485S      品牌：CST       原厂货号：4485S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PTPmu小鼠单抗检测内源性总PTPmu蛋白水平。此抗体不与其他受体酪氨酸磷酸酶交叉反应。单克隆抗体（同中型：IgG2a）由合成肽段免疫小鼠产生，对应人PTPmu氨基末端残基。受体酪氨酸磷酸酶PTPmu有一个粘附分子的胞外片段，一个MEM结构域，一个Ig结构域，四个纤连蛋白III样(FN III)重复（1,2）。PTPmu蛋白水解式剪切成两个非共价的片段：一个是胞外结构域，另一个包括跨膜和胞内催化结构域。两个片段大约100kDa（3）。胞外结构域以亲同种抗原的，Ca2+独立的方式调节细胞间粘附（1,2）。PTPmu与多种cadherins相关联（4）。在人前列腺癌中能恢复E-cadherin依赖的粘附，对于N-cadherin调节的轴突产物是必需的（5,6）磷酸酶活性似乎杜宇后者功能所以必需，但对前者可有可无（5,6）。PTPmu也与支架蛋白的相关并招募支架蛋白（7）。PKCdelta和 src似乎涉及此过程（6，7）。

1. Gebbink, M. F. et al. (1993) J. Biol. Chem. 268, 16101-16104.
2. Brady-Kalnay, S.M. and Tonks, N.K. (1994) J. Biol. Chem. 269, 28472-28477.
3. Brady-Kalnay, S. M. et al. (1998) J. Cell Biol. 141, 287-296.
4. Hellberg, C. B. et al. (2002) J. Biol. Chem. 277, 11165-11173.
5. Burden-Gulley, S.M. and Brady-Kalnay, S.M. (1999) J. Cell Biol. 144, 1323-1336.
6. Mourton, T. et al. (2001) J. Biol. Chem. 276, 14896-14901.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

PTPmu Mouse mAb detects endogenous levels of total PTPmu protein. This antibody does not cross react with other receptor tyrosine pho sphatases.

Source / Purification

Monoclonal antibody (isotype: IgG2a) is produced by immunizing mice with a synthetic peptide corresponding to the amino-terminal residues of human PTPmu.

Background

Receptor tyrosine phosphatase PTPmu has an extracellular segment characteristic of adhesion molecules: an MEM domain, an Ig domain and four fibronectin III like (FN III) repeats (1,2). PTPmu is proteolytically cleaved into two noncovalently associated fragments: one is the extracellular domain, the other includes the transmembrane and the intracellular catalytic domains. Both fragments are approximately 100 kDa (3). The extracellular domain mediates cell-cell adhesion in a homophilic, Ca2+ independent manner (1,2). PTPmu associates with multiple cadherins (4). It is able to restore E-cadherin-dependent adhesion in human prostate cancer, and is required for N-cadherin-mediated neurite outgrowth (5,6). The phosphatase activity seems to be essential for the latter function but is dispensable for the former (5,6). PTPmu also associates with and recruits a scaffold protein, RACK (receptor for activated protein C kinase), to cell-cell contact sites (7). Both PKCdelta and src seem to be involved in this process (6,7).

1. Gebbink, M. F. et al. (1993) J. Biol. Chem. 268, 16101-16104.
2. Brady-Kalnay, S.M. and Tonks, N.K. (1994) J. Biol. Chem. 269, 28472-28477.
3. Brady-Kalnay, S. M. et al. (1998) J. Cell Biol. 141, 287-296.
4. Hellberg, C. B. et al. (2002) J. Biol. Chem. 277, 11165-11173.
5. Burden-Gulley, S.M. and Brady-Kalnay, S.M. (1999) J. Cell Biol. 144, 1323-1336.
6. Mourton, T. et al. (2001) J. Biol. Chem. 276, 14896-14901.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody