Phospho-PTPα (Tyr789) Antibody

• 产品编号：CST-4481S      品牌：CST       原厂货号：4481S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PTPα (Tyr789) Antibody兔多抗能检测内源性酪氨酸（789位点）磷酸化的PP2C-α蛋白水平。该抗体不能与其他磷酸化的受体酪氨酸磷酸酶发生交叉反应。该多克隆抗体通过使用与人源 PTPα.蛋白酪氨酸（789位点）周围残基相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。PTPα (PTPRA)是一种跨膜受体酪氨酸磷酸酶，参与Src家族激酶的调节，包括G2到有丝分裂入口点。两个已经鉴别出来的剪切突变体在细胞外区域中的大小不同，较短的结构形式呈现遍在化表达，而较大蛋白的分布范围有更多的限制(1)。PTPα的胞质区域包含两个假定的催化区域。其中一个磷酸酶区域（D1）呈现催化活性，然而另外一个D2可能通过受体二聚物的形成而调节磷酸酶的活性(2,3)。PTPα是Src和 Src家族激酶生理学调节子(4)。PTPα羧端酪氨酸（789位点）的结构性磷酸化对Src蛋白酪氨酸（527位点）的去磷酸化十分重要。该位点PTPα的磷酸化允许Grb2抑制子的结合，限制了PTPα 对Src的活化(5,6)。PKC调节的PTP丝氨酸（180、204位点）的磷酸化也提高了PTPα磷酸酶的活性 (7)。

1. Kapp, K. et al. (2007) Genes Cells 12, 63-73.
2. Blanchetot, C. et al. (2002) J Biol Chem 277, 47263-9.
3. Krueger, N.X. et al. (1990) EMBO J 9, 3241-52.
4. den Hertog, J. et al. (1993) EMBO J 12, 3789-98.
5. Zheng, X.M. et al. (2000) EMBO J 19, 964-78.
6. Zheng, X.M. et al. (2002) J Biol Chem 277, 21922-9.
7. Tracy, S. et al. (1995) J Biol Chem 270, 10587-94.

原厂资料：

Specificity / Sensitivity

Phospho-PTPα (Tyr789) Antibody detects endogenous levels of PTPα only when phosphorylated at Tyr789. This antibody does not cross-react with other phosphorylated receptor tyrosine phosphatases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr789 of human PTPα. Antibodies are purified by protein A and peptide affinity chromatography.

Background

PTPα (PTPRA) is a transmembrane receptor tyrosine phosphatase implicated in the regulation of Src family kinases during the G2 to mitosis entry point. Two identified splice variants differ in the size of the extracellular region; the shorter form appears to be ubiquitously expressed while the larger protein is more limited in distribution (1). The cytoplasmic region of PTPα contains two putative catalytic domains. One phosphatase domain (D1) exhibits catalytic activity while the other (D2) may regulate phosphatase activity by allowing receptor dimer formation (2,3). PTPα is a physiological regulator of Src and Src family kinases (4). Constitutive phosphorylation of the carboxy-terminal Tyr789 of PTPα is essential for dephosphorylation of Src at Tyr527. Phosphorylation of PTPα at this residue also allows binding of the Grb2 inhibitor, restricting PTPα activation of Src (5,6). PKC-mediated phosphorylation of the PTP at Ser180 and Ser204 also increases PTPα phosphatase activity (7).

1. Kapp, K. et al. (2007) Genes Cells 12, 63-73.
2. Blanchetot, C. et al. (2002) J Biol Chem 277, 47263-9.
3. Krueger, N.X. et al. (1990) EMBO J 9, 3241-52.
4. den Hertog, J. et al. (1993) EMBO J 12, 3789-98.
5. Zheng, X.M. et al. (2000) EMBO J 19, 964-78.
6. Zheng, X.M. et al. (2002) J Biol Chem 277, 21922-9.
7. Tracy, S. et al. (1995) J Biol Chem 270, 10587-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.