RagD Antibody

• 产品编号：CST-4470S      品牌：CST       原厂货号：4470S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RagD Antibody可以检测内源性RagD总蛋白。该多克隆抗体是由人源的RagD蛋白序列相对应的合成肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。mTORC1激酶复合物是调节细胞生长的一个关键组分（1,2）。活性由能量水平，生长因子和氨基酸调节（3,4）。最近的研究发现，RagA，RagB，RagC和RagD，这四个相关的GTPases与mTORC1中的raptor互作（1,2）。这些互作是mTORC1响应氨基酸信号活化所必须的和有效的（1,2）。RagD 是差异表达在人类黑素瘤细胞系上的一个基因，已经被认为未来诊断与治疗研究中非常可行的目标靶基因（5）。

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
5. de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61.

原厂资料：

Specificity / Sensitivity

RagD Antibody detects endogenous levels of total RagD protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagD protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

mTORC1 kinase complex is a critical component in regulating cell growth (1,2). Its activity is modulated by energy levels, growth factors and amino acids (3,4). Recent studies found that RagA, RagB, RagC and RagD, the four related GTPases, interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered as a viable target for further diagnostic and therapeutic study (5).

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
5. de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.