Ran Antibody

• 产品编号：CST-4462S      品牌：CST       原厂货号：4462S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Ran Antibody检测内源性Ran总蛋白。该抗体不能与Ras家族的其它小GTPases发生交叉反应。通过人工合成人源Ran蛋白Arg29和Asn143位点相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Ran蛋白是Ras家族的一个小GTPase，它在真核细胞的空间和时间的组织上起着关键的作用。在有丝分裂间期，Ran-GDP定位于细胞质，并且Ran-GDP朝着细胞核方向。Ran蛋白的定位的极化确定了它在细胞核转运中的作用(1)。在有丝分裂期，Ran-GTP是核染色质相关的，在哪里可促进纺锤体的装配和细胞核核膜的形成(1,2)。在S期，Ran-GTP与MCM解旋酶有关联并且抑制它，在细胞周期中这确保染色体DNA精确的复制(3)。

1. Quimby, B.B. and Dasso, M. (2003) Curr. Opin. Cell Biol. 15, 338-344.
2. Hetzer, M. et al. (2002) Nat. Cell Biol. 4, 177-184.
3. Yamaguchi, R. and Newport, J. (2003) Cell 113, 115-125.

原厂资料：

Specificity / Sensitivity

Ran Antibody detects endogenous levels of total Ran protein. The antibody does not cross-react with other small GTPases of the Ras family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3).

1. Quimby, B.B. and Dasso, M. (2003) Curr. Opin. Cell Biol. 15, 338-344.
2. Hetzer, M. et al. (2002) Nat. Cell Biol. 4, 177-184.
3. Yamaguchi, R. and Newport, J. (2003) Cell 113, 115-125.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody