Nna1 Antibody

• 产品编号：CST-4456S      品牌：CST       原厂货号：4456S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nna1 Antibody 识别内源性的Nna1总蛋白。该多克隆抗体由合成的人类Nna1蛋白Asp1175位点附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。被axotomy1（Nna1, AGTPBP1或cytosolic carboxypeptidase-1）诱导的神经系统核蛋白质锌carboxypeptidases有关，包含一个ATP/GTP结合模体，一个基本和双边的核定位信号。轴突切断可快速诱导运动神经元的Nna1表达，再连接可下调其表达。Nna1的表达在分化中的神经元上表达丰富，在CNS部分增生区域缺如（1）。此外，Nna1基因突变可导致以浦肯野细胞变性为特点的成人发病的神经退行性变，这暗示Nna1可能在神经变性和再生机制中发挥作用（2）。

1. Harris, A. et al. (2000) Mol Cell Neurosci 16, 578-96.
2. Fernandez-Gonzalez, A. et al. (2002) Science 295, 1904-6.

原厂资料：

Specificity / Sensitivity

Nna1 Antibody detects endogenous levels of total Nna1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1175 of human Nna1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nervous system nuclear protein induced by axotomy 1 (Nna1, AGTPBP1 or cytosolic carboxypeptidase-1) is related to zinc carboxypeptidases and contains an ATP/GTP binding motif, a basic and a bipartite nuclear localization signal. Nna1 expression is rapidly induced in motor neurons following axotomy and down-regulated following reinnervation, which is consistent with abundant Nna1 expression in differentiating neurons and the absence of Nna1 in CNS proliferative zones (1). Furthermore, Purkinje cell degeneration is characterized by adult onset neurodegeneration resulting from Nna1 gene mutations, implicating Nna1 in mechanisms common to degeneration and regeneration (2).

1. Harris, A. et al. (2000) Mol Cell Neurosci 16, 578-96.
2. Fernandez-Gonzalez, A. et al. (2002) Science 295, 1904-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody