CCTα Antibody

• 产品编号：CST-4454S      品牌：CST       原厂货号：4454S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CCTα Antibody用于检测内源性CCTα蛋白水平。多克隆抗体通过用对应于人源CCTα蛋白氮端序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。CTP:磷酸胆碱胞苷酸转移酶（CCT）是调控卵磷脂生物合成中CDP－胆碱途径的关键酶。该酶在哺乳动物中有三种同工酶，包括CCTα, CCTβ2, 和CCTβ3 (1,2)。CCTα为在大多数组织中表达的形式（3）。CCTα对肺泡上皮细胞合成与分泌表面活性剂是必需的，此外，它对维持正常的卵磷脂水平，从而调节肝脏细胞脂蛋白合成与分泌是至关重要的（4,5）。在巨噬细胞激活后分泌细胞因子过程中，CCTα还是膜泡合成的一个主要组分。CCTα的单泛素化会阻止其入核，并被溶酶体降解（7）。

1. Jackowski, S. and Fagone, P. (2005) J Biol Chem 280, 853-6.
2. Clement, J.M. and Kent, C. (1999) Biochem Biophys Res Commun 257, 643-50.
3. Karim, M. et al. (2003) Biochim Biophys Acta 1633, 1-12.
4. Tian, Y. et al. (2007) Mol Cell Biol 27, 975-82.
5. Jacobs, R.L. et al. (2004) J Biol Chem 279, 47402-10.
6. Tian, Y. et al. (2008) J Cell Biol 181, 945-57.
7. Chen, B.B. and Mallampalli, R.K. (2009) Mol Cell Biol 29, 3062-75.

原厂资料：

Specificity / Sensitivity

CCTα Antibody detects endogenous levels of total CCTα protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CCTα protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

CTP:phosphocholine cytidylyltransferase (CCT) is a critical enzyme that regulates the CDP-choline pathway for the biosynthesis of phosphatidylcholine. Three distinct CCT isoforms are found in mammals, including CCTα, CCTβ2, and CCTβ3 (1,2). CCTα is the major isoform that is expressed in most tissues (3). CCTα is essential in the synthesis and secretion of surfactant by alveolar epithelial cells and is important in maintaining the phosphatidylcholine level that regulates lipoprotein assembly and secretion in hepatocytes (4,5). CCTα is a major component in membrane biogenesis during cytokine secretion by stimulated macrophages (6). Monoubiquitination of CCTα prevents it from entering the nucleus and leads to its degradation by lysosome (7).

1. Jackowski, S. and Fagone, P. (2005) J Biol Chem 280, 853-6.
2. Clement, J.M. and Kent, C. (1999) Biochem Biophys Res Commun 257, 643-50.
3. Karim, M. et al. (2003) Biochim Biophys Acta 1633, 1-12.
4. Tian, Y. et al. (2007) Mol Cell Biol 27, 975-82.
5. Jacobs, R.L. et al. (2004) J Biol Chem 279, 47402-10.
6. Tian, Y. et al. (2008) J Cell Biol 181, 945-57.
7. Chen, B.B. and Mallampalli, R.K. (2009) Mol Cell Biol 29, 3062-75.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody