Caspase-4 Antibody

• 产品编号：CST-4450S      品牌：CST       原厂货号：4450S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Caspase-4 Antibody能够检测内源性caspase-4总蛋白。如之前报道，研究发现caspase-4 形成40 kDa和32 kDa的中间体（7）。该抗体不与其它 caspases 发生交叉反应。该多克隆抗体是采用合成的在人源caspase-4蛋白p20亚基的Ile125周围残基相应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。Caspase-4 (TX/ICH-2/ICErelII)是caspase家族的一员，在细胞凋亡的执行和炎症细胞因子的激活中发挥了关键作用（1-3）。已经在除了大脑外的大多数组织中观察到caspase-4的表达，在胎盘、肺、脾和外周血淋巴细胞（PBL）中的表达水平最高。最初发现Caspase-4是作用于Fas介导的细胞凋亡（4）。一些caspases（包括caspase-4、caspase-5和小鼠caspase-11和-12）与caspase-1的关系密切，过表达时能够诱导细胞凋亡，但更重要的特征是炎性细胞因子的蛋白水解激活（5）。Caspase-4与TRAF6 的协作，参与了THP-1细胞中脂多糖诱导的炎性细胞因子IL-8和MIP1的产生（6）。Caspase-4和小鼠caspase-12定位到内质网上（ER），并且可能由诱导的ER应激的药物激活（7），至少有一项研究表明，caspase-4和caspase-12对于ER应激诱导的细胞凋亡不是必不可少的（8）。

1. Faucheu, C. et al. (1995) EMBO J 14, 1914-22.
2. Kamens, J. et al. (1995) J Biol Chem 270, 15250-6.
3. Munday, N.A. et al. (1995) J Biol Chem 270, 15870-6.
4. Kamada, S. et al. (1997) Oncogene 15, 285-90.
5. Martinon, F. and Tschopp, J. (2007) Cell Death Differ 14, 10-22.
6. Lakshmanan, U. and Porter, A.G. (2007) J Immunol 179, 8480-90.
7. Hitomi, J. et al. (2004) J Cell Biol 165, 347-56.
8. Obeng, E.A. and Boise, L.H. (2005) J Biol Chem 280, 29578-87.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Caspase-4 Antibody detects endogenous levels of total caspase-4 protein. Processing intermediate forms of caspase-4 are observed at 40 kDa and 32 kDa as previously reported (7). The antibody does not cross-react with other caspases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile125 within the p20 subunit of human caspase-4 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Caspase-4 (TX/ICH-2/ICErelII) is a member of the caspase family of proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Expression of caspase-4 has been observed in most tissues except brain, with highest levels in placenta, lung, spleen, and peripheral blood lymphocytes (PBL). Caspase-4 was originally found to contribute to Fas-mediated apoptosis (4). Several caspases (including caspase-4, caspase-5, and mouse caspase-11 and -12) are most closely related to caspase-1 and are capable of inducing apoptosis when over-expressed but are better characterized in the proteolytic activation of inflammatory cytokines (5). Caspase-4 associates with TRAF6 and is involved in the LPS inducible production of inflammatory cytokines IL-8 and MIP1 in THP-1 cells (6). While caspase-4 and mouse caspase-12 localize to the endoplasmic reticulum (ER) and may be activated by drugs that induce ER-stress (7), at least one study suggests that caspase-4 and caspase-12 are not essential for the ER-stress induced apoptosis (8).

1. Faucheu, C. et al. (1995) EMBO J 14, 1914-22.
2. Kamens, J. et al. (1995) J Biol Chem 270, 15250-6.
3. Munday, N.A. et al. (1995) J Biol Chem 270, 15870-6.
4. Kamada, S. et al. (1997) Oncogene 15, 285-90.
5. Martinon, F. and Tschopp, J. (2007) Cell Death Differ 14, 10-22.
6. Lakshmanan, U. and Porter, A.G. (2007) J Immunol 179, 8480-90.
7. Hitomi, J. et al. (2004) J Cell Biol 165, 347-56.
8. Obeng, E.A. and Boise, L.H. (2005) J Biol Chem 280, 29578-87.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody