Autophagy Antibody Sampler Kit

• 产品编号：CST-4445S      品牌：Cell Signaling Technology       原厂货号：4445S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Autophagy Antibody Sampler 试剂盒中的每个抗体都能够检测内源性的目的蛋白。Atg5和Atg12兔单抗能够识别Atg12-Atg5的复合物。LC3A和LC3B 兔单抗可以与其它形式的LC3发生交叉反应。在进行Western blot实验时，LC3B兔单抗对于II型的LC3B具有更强的反应性。这些单克隆抗体通过合成肽免疫动物获得，这些合成肽的序列分别与人源的Beclin-1蛋白Thr72、人源Atg5蛋白的Pro187，人源Atg12的Ser36或者是人源LC3A和LC3B的氨基末端周围残基相一致。这些多克隆抗体通过合成肽免疫动物获得，这些合成肽与人源Atg3和Atg7氨基末端附近的残基相一致。多克隆抗体通过蛋白A和多肽亲和色谱法纯化。Autophagy Antibody Sampler 试剂盒提供了一种经济有效的方法，研究细胞中自噬的分子机制。这种试剂盒包含了足够的一抗和二抗，可进行4次Western blot试验。

原厂资料：

Specificity / Sensitivity

Each antibody in the Autophagy Antibody Sampler Kit detects its respective target at endogenous levels. Both the Atg5 and Atg12 rabbit monoclonal antibodies recognize the Atg12-Atg5 conjugate. The LC3A and LC3B rabbit monoclonal antibodies may cross-react with other LC3 isoforms. The LC3B rabbit monoclonal antibody has stronger reactivity with the type II form of LC3B by western immunoblot.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Thr72 of human Beclin-1, Pro187 of human Atg5, Ser36 of human Atg12, or near the amino termini of human LC3A and LC3B. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino-termini of human Atg3 and Atg7. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Autophagy Antibody Sampler Kit provides an economical means to investigate the molecular machinery of autophagy within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibodi