Phospho-Stat2 (Tyr690) Antibody

• 产品编号：CST-4441P      品牌：Cell Signaling Technology       原厂货号：4441P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Stat2 Antibody只能检测内源的在Tyr690位点磷酸化的Stat2蛋白。此抗体不与对应的酪氨酸磷酸化的Stat家族的其它蛋白交叉反应。此多克隆抗体是通过合成人源对应的Stat2 Tyr690位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。Stat2 (113kDa),最初是从用alpha干扰素处理的细胞核中提纯，它对类型I干扰素IFN-alpha/beta的诱导的转录响应是非常重要的(1,2)。 针对Stat2基因敲除的小鼠对病毒的感染呈现出易感性对类型I干扰素的反应发生改变(3)。Stat2的激活是在IFN-alpha/beta的诱导下通过受体结合到Jak激酶上而在 Tyr690位点快速被磷酸化(4)。与其它的Stat蛋白不同, Stat2 不形成同源二聚体，激活的Stat2与Stat1形成异源二聚体并定位到细胞核内。在细胞核内它与DNA结合蛋白p48结合并形成转录激活复合体——干扰素刺激因子3(ISGF3)，介导从ISRE开始的转录(5)。

1. Fu, X.Y. et al. (1992) Proc Natl Acad Sci U S A 89, 7840-3.
2. Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
3. Park, C. et al. (2000) Immunity 13, 795-804.
4. Improta, T. et al. (1994) Proc Natl Acad Sci U S A 91, 4776-80.
5. Horvath, C.M. et al. (1996) Mol Cell Biol 16, 6957-64.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Bovine

Specificity / Sensitivity

Phospho-Stat2 Antibody detects endogenous levels of human Stat2 only when phosphorylated at Tyr690. The antibody does not significantly cross-react with corresponding phospho-tyrosine sites on other Stat proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr690 of human Stat2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Stat2 (113-kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5).

1. Fu, X.Y. et al. (1992) Proc Natl Acad Sci U S A 89, 7840-3.
2. Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
3. Park, C. et al. (2000) Immunity 13, 795-804.
4. Improta, T. et al. (1994) Proc Natl Acad Sci U S A 91, 4776-80.
5. Horvath, C.M. et al. (1996) Mol Cell Biol 16, 6957-64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.