PRMT4/CARM1 Antibody

• 产品编号：CST-4438S      品牌：CST       原厂货号：4438S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PRMT4/CARM1 Antibody能够检测内源性PRMT4/CARM1的总蛋白水平（亚型1）。该抗体不能与PRMT4/CARM1蛋白2型亚基发生相互作用。该抗体不能与其它PRMT蛋白发生相互作用。通过合成的与人源PRMT4/CARM1蛋白羧基末端周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Protein arginine N-methyltransferase 4 (PRMT4)又称为coactivator-associated arginine methyltransferase 1 (CARM1)，它是蛋白质精氨酸甲基转移酶(PRMT)蛋白家族的一个成员，它以S-腺苷-甲硫氨酸(S-adenosylmethionine,AdoMet)为甲基供体，把甲基转移到蛋白质精氨酸胍基的氮原子上 (1)。PRMT蛋白有两种形式。当两种形式都能催化催化单甲基精氨酸的形成时，I 型PRMTs (PRMT1, 3, 4, and 6)增加一个附加的甲基能产生一个不对称双甲基精氨酸，以及II型PRMTs (PRMT 5 and 7)产生对称型双甲基精氨酸(1)。单甲基精氨酸而不是双甲基精氨酸，它能通过由酶例如PADI4催化deimination过程去转换成瓜氨酸(2)。许多PRMTs包括PRMT1，在甘氨酸 - 精氨酸富足（GAR）蛋白区域，例如RGG, RG, and RXR重复序列中发现甲基化精氨酸残基(1)。然而，PRMT4/CARM1 and PRMT5可以使PGM (proline-, glycine-, methionine-rich) 结构中精氨酸甲基化(3)。PRMT4能使组蛋白H3的Arg2、17和26位点甲基化，并且通过细胞核受体蛋白与p300/CBP协同调节去提高转录激活(4)。此外，PRMT4能使许多非组蛋白甲基化，包括转录共激活因子(p300/CBP, SRC-3) (5,6,7,8)、剪切因子(SmB, CA150, SAP49, UIC) (3)、RNA结合蛋白(PABP1, Sam68, HuD, HuR) (9,10,11)和thymocyte cyclic AMP-regulated phosphoprotein (TARPP) (12)，这认为另外的功能是转录调控、mRNA加工和胸腺细胞成熟。通过PRMT4使剪切因子CA150的甲基化有助于与SMN蛋白Tudor区域相互作用，这认为PRMT4蛋白在脊髓性肌萎缩症中起着重要作用(3)。

1. Bedford, M.T. and Richard, S. (2005) Mol. Cell 18, 263-272.
2. Wang, Y. et al. (2004) Science 306, 279-283.
3. Cheng, D. et al. (2007) Mol. Cell 25, 71-83.
4. Chen, D. et al. (2000) J. Biol. Chem. 275, 40810-40816.
5. Lee, Y.H. et al. (2005) Proc. Natl. Acad. Sci. USA 102, 3611-3616.
6. Xu, W. et al. (2001) Science 294, 2507-2511.
7. Naeem, H. et al. (2007) Mol. Cell Biol. 27, 120-134.
8. Feng, Q. et al. (2006) Mol. Cell Biol. 26, 7846-7857.
9. Lee, J. and Bedford, M.T. (2002) EMBO Rep. 3, 268-273.
10. Côté, J. et al. (2003) Mol. Biol. Cell 14, 274-287.
11. Fujiwara, T. et al. (2006) Mol. Cell Biol. 26, 2273-2285.
12. Kim, J. et al. (2004) J. Biol. Chem. 279, 25339-25344.

原厂资料：

Specificity / Sensitivity

PRMT4/CARM1 Antibody detects endogenous levels of PRMT4/CARM1 protein (isoform 1). The antibody will not detect isoform 2 of PRMT4/CARM1. This antibody does not cross-react with other PRMT proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human PRMT4/CARM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein arginine N-methyltransferase 4 (PRMT4), also known as coactivator-associated arginine methyltransferase 1 (CARM1), is a member of the protein arginine N-methyltransferase (PRMT) family of proteins, which catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (1). There are two types of PRMT proteins. While both types catalyze the formation of mono-methyl arginine, type I PRMTs (PRMT1, 3, 4 and 6) add an additional methyl group to produce asymmetric di-methyl arginine and type II PRMTs (PRMT 5 and 7) produce symmetric di-methyl arginine (1). Mono-methyl arginine, but not di-methyl arginine, can be converted to citrulline through deimination performed by enzymes such as PADI4 (2). Most of the PRMTs methylate arginine residues found within glycine-arginine rich (GAR) domains of proteins, such as RGG, RG and RXR repeats (1). However, PRMT4/CARM1 and PRMT5 instead methylate arginine residues within PGM (proline-, glycine-, methionine-rich) motifs (3). PRMT4 methylates Arg2, 17 and 26 of histone H3 and cooperates synergistically with p300/CBP and p160 coactivators to enhance transcriptional activation by nuclear receptor proteins (4). In addition, PRMT4 methylates many non-histone proteins, including transcriptional coactivators (p300/CBP, SRC-3) (5,6,7,8), splicing factors (SmB, CA150, SAP49, UIC) (3), RNA binding proteins (PABP1, Sam68, HuD, HuR) (9,10,11), and thymocyte cyclic AMP-regulated phosphoprotein (TARPP) (12), suggesting additional functions in transcriptional regulation, mRNA processing and thymocyte maturation. Methylation of the splicing factor CA150 by PRMT4 facilitates an interaction with the Tudor domain of SMN, suggesting a role for PRMT4 in spinal muscular atrophy (3).

1. Bedford, M.T. and Richard, S. (2005) Mol. Cell 18, 263-272.
2. Wang, Y. et al. (2004) Science 306, 279-283.
3. Cheng, D. et al. (2007) Mol. Cell 25, 71-83.
4. Chen, D. et al. (2000) J. Biol. Chem. 275, 40810-40816.
5. Lee, Y.H. et al. (2005) Proc. Natl. Acad. Sci. USA 102, 3611-3616.
6. Xu, W. et al. (2001) Science 294, 2507-2511.
7. Naeem, H. et al. (2007) Mol. Cell Biol. 27, 120-134.
8. Feng, Q. et al. (2006) Mol. Cell Biol. 26, 7846-7857.
9. Lee, J. and Bedford, M.T. (2002) EMBO Rep. 3, 268-273.
10. Côté, J. et al. (2003) Mol. Biol. Cell 14, 274-287.
11. Fujiwara, T. et al. (2006) Mol. Cell Biol. 26, 2273-2285.
12. Kim, J. et al. (2004) J. Biol. Chem. 279, 25339-25344.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.