Anti-rabbit IgG (H+L)F(ab)2 Fragment (Alexa Fluor? 555 Conjugate)

• 产品编号：CST-4413S      品牌：CST       原厂货号：4413S
• 产品分类：抗体 > 二抗 > 新型荧光染料标记
• 应用分类：

描述：

F(ab’)2片段是采用羊抗体制备的，已经吸附了人血清、鼠血清、浆细胞瘤/杂交瘤蛋白和纯化的人类病变蛋白。Anti- Rabbit IgG (H+L) F(ab’)2 Fragment在最佳条件下结合了 Alexa Fluor ® 555荧光染料，浓度为2mg/ml. F(ab’)2片段可以减少通过Fc受体的非特异性结合。本产品已经过优化，可以作为二抗应用于免疫荧光中。抗体的最佳稀释度取决于每一个应用。然而，1:1000的终稀释度在免疫荧光分析中可以得到好的结果。荧光抗IgG结合物适用于流式细胞术和免疫荧光。CST公司的严格的质控程序保证了每一个结合物具有最佳的特异性和荧光强度。

原厂资料：

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

Description

Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Applications

This product has been optimized for use as a secondary antibody in immunofluorescent applications.

Directions for Use

The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescentapplications.

Background

Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

注意事项：

Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium
chloride, 2 mM EDTA, 1% glycerol, 5 mM sodium azide, pH 7.5.
Store at 4°C. Protect from light. Do not freeze.