FoxP1 (D35D10) XP? Rabbit mAb

• 产品编号：CST-4402P      品牌：Cell Signaling Technology       原厂货号：4402P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FoxP1 (D35D10) XP^® Rabbit mAb兔单抗可以识别内源性的总FoxP1蛋白。合成与人源FoxP1蛋白的邻近584位亮氨酸氨基酸残基序列一致的肽段，免疫动物可以获得单克隆抗体。叉头盒（Fox）蛋白是进化保守转录因子家族之一，含有一个叉头盒或翼型螺旋DNA结合结构域的序列（1）。人类基因组含有43个叉头蛋白，并被分为亚家族。FoxP亚家族有四个成员FoxP1 到 FoxP4,它们广泛表达，并在器官发育，免疫反应和癌症病例中起重要作用（2-4）。FoxP亚家族有一些非典型的Fox蛋白的特点，就是它们的叉头结构域定位在羧基末端区域，它们含有的基序促进同源和异源二聚化。FoxP蛋白经常起着转录抑制因子功能（4,5）..FoxP1对心脏瓣膜和B细胞发育起重要作用，在小鼠中靶向敲出FoxP1会导致胚胎在E14,5时死亡，并有严重心脏血流发育和心内膜垫形态发生以及肌细胞增殖成熟的缺失（6），FoxP1基因坐落在染色体3p14.1，多种肿瘤中在这一区域的杂合子丢失，显示了Foxp1肿瘤抑制因子的作用（7）。另外，FoxP1在各种B细胞恶性肿瘤中高表达，高频率涉及染色体异位，暗示FoxP1也可能是癌基因（8,9）.

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
3. Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
4. Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
5. Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
6. Wang, B. et al. (2004) Development 131, 4477-87.
7. Banham, A.H. et al. (2001) Cancer Res 61, 8820-9.
8. Wlodarska, I. et al. (2005) Leukemia 19, 1299-305.
9. Goatly, A. et al. (2008) Mod Pathol 21, 902-11.

原厂资料：

Specificity / Sensitivity

FoxP1 (D35D10) XP^® Rabbit mAb detects endogenous levels of total FoxP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu584 of human FoxP1 protein.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 to FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region, and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).FoxP1 plays an important role in cardiac valve and B cell development. Targeted deletion of FoxP1 in mice results in embryonic death at E14.5 with severe defects in cardiac outflow development, endocardial cushion morphogenesis, and myocyte proliferation and maturation (6). The FoxP1 gene is located on chromosome 3p14.1 and loss of heterozygosity of this region in multiple cancer types suggests a role for Foxp1 as a tumor suppressor (7). On the other hand, FoxP1 is highly expressed in a variety of B cell malignancies and is frequently involved in chromosome translocation, suggesting that FoxP1 may also act as an oncogene (8,9).

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
3. Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
4. Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
5. Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
6. Wang, B. et al. (2004) Development 131, 4477-87.
7. Banham, A.H. et al. (2001) Cancer Res 61, 8820-9.
8. Wlodarska, I. et al. (2005) Leukemia 19, 1299-305.
9. Goatly, A. et al. (2008) Mod Pathol 21, 902-11.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody