MTSS1 (N747) Antibody

• 产品编号：CST-4386S      品牌：CST       原厂货号：4386S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MTSS1 (N747) Antibody检测内源性MTSS1总蛋白。通过人工合成人源MTSS1蛋白Asn747位点相应多肽片段去免疫动物从而制备出多克隆抗体。通过多肽亲和层析纯化抗体。Metastasis suppressor 1 (MTSS1)蛋白是一个多功能支架蛋白，起初使用一个差异显示技术发现该蛋白，而该技术用于揭示在膀胱癌细胞系中蛋白表达的差异(1,2)。MTSS1 (又称为Missing in Metastasis或MIM)蛋白是一个细胞骨架重构蛋白，它包含一个C端WH2 actin结合域(1,3)。一个IMD (IRSp53/MIM homology)结构域的存在允许细胞内MTSS1蛋白去诱导F-actin的捆绑和丝状伪足形成 (4)。MTSS1蛋白结合和激活Rac蛋白，而Rac蛋白是已知促进丝状伪足和板状伪足的形成(5)。tyrosine phosphatase δ (PTPRD)受体是与MTSS1蛋白有联系，并且对MTSS1蛋白依赖的细胞骨架改变是需要的(6,7)。MTSS1蛋白是一个Shh反应基因，它能帮助调节Gli转录活性(8)。通过DNA甲基化调节MTSS1基因的转录(9)。

1. Lee, Y.G. et al. Neoplasia 4, 291-4.
2. Machesky, L.M. and Johnston, S.A. (2007) J Mol Med 85, 569-76.
3. Mattila, P.K. et al. (2003) J Biol Chem 278, 8452-9.
4. Yamagishi, A. et al. (2004) J Biol Chem 279, 14929-36.
5. Bompard, G. et al. (2005) J Cell Sci 118, 5393-403.
6. Woodings, J.A. et al. (2003) Biochem J 371, 463-71.
7. Gonzalez-Quevedo, R. et al. (2005) J Cell Biol 168, 453-63.
8. Callahan, C.A. et al. (2004) Genes Dev 18, 2724-9.
9. Utikal, J. et al. (2006) Int J Cancer 119, 2287-93.

原厂资料：

Specificity / Sensitivity

MTSS1 (N747) Antibody detects endogenous levels of total MTSS1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Asn747 of human MTSS1. Antibodies are purified by peptide affinity chromatography.

Background

Metastasis suppressor 1 (MTSS1) is a multi-functional scaffold protein that was initially discovered using a differential display technique that uncovered proteins missing from bladder cancer cell lines (1,2). MTSS1 (also known as Missing in Metastasis or MIM) is a cytoskeletal remodeling protein that contains a C-terminal WH2 actin-binding motif (1,3). Presence of an IMD (IRSp53/MIM homology) domain allows MTSS1 to induce F-actin bundling and filopodia formation in cells (4). MTSS1 binds to and activates Rac, a protein known to promote the formation of filopodia and lamellipodia (5). The receptor tyrosine phosphatase δ (PTPRD) is associated with MTSS1 and is required for MTSS1-dependent cytoskeletal change (6,7). MTSS1 is a Shh responsive gene that can help regulate Gli transcriptional activity (8). Transcription of MTSS1 is regulated by DNA methylation (9).

1. Lee, Y.G. et al. Neoplasia 4, 291-4.
2. Machesky, L.M. and Johnston, S.A. (2007) J Mol Med 85, 569-76.
3. Mattila, P.K. et al. (2003) J Biol Chem 278, 8452-9.
4. Yamagishi, A. et al. (2004) J Biol Chem 279, 14929-36.
5. Bompard, G. et al. (2005) J Cell Sci 118, 5393-403.
6. Woodings, J.A. et al. (2003) Biochem J 371, 463-71.
7. Gonzalez-Quevedo, R. et al. (2005) J Cell Biol 168, 453-63.
8. Callahan, C.A. et al. (2004) Genes Dev 18, 2724-9.
9. Utikal, J. et al. (2006) Int J Cancer 119, 2287-93.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody