Gremlin Antibody

• 产品编号：CST-4383S      品牌：CST       原厂货号：4383S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Gremlin抗体识别内源性的总gremlin蛋白。合成与人gremlin蛋白邻近Val170氨基酸残基序列一致的肽段，免疫动物获得多克隆抗体。抗体由蛋白A和肽段亲和层析法纯化。Gremlin是骨形成蛋白的一种分泌型拮抗物，在发育过程中扮演重要角色。基因打靶构建的gremlin缺陷小鼠在出生后迅速死亡，源于肾脏缺失和肺间隔的缺陷（1-3）。Gremlin同时也在心脏形成和血管生成过程中发挥作用（4，5）。TGF-β可以在病理条件下诱导gremlin表达。Gremlin与TGF-β共同位于小管间质性纤维化伴随着慢性异体移植肾病变，并且在体内糖尿病病变中表达（6-8）。Gremlin在癌症伴随的基质细胞中广泛表达并且促进肿瘤细胞增殖（9）

1. Zúñiga, A. et al. (1999) Nature 401, 598-602.
2. Zuniga, A. et al. (2004) Genes Dev 18, 1553-64.
3. Michos, O. et al. (2004) Development 131, 3401-10.
4. Stabile, H. et al. (2007) Blood 109, 1834-40.
5. Kami, D. et al. (2008) PLoS ONE 3, e2407.
6. Carvajal, G. et al. (2008) Transplant Proc 40, 734-9.
7. McMahon, R. et al. (2000) J Biol Chem 275, 9901-4.
8. Dolan, V. et al. (2005) Am J Kidney Dis 45, 1034-9.
9. Sneddon, J.B. et al. (2006) Proc Natl Acad Sci USA 103, 14842-7.

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原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Rat

Specificity / Sensitivity

Gremlin Antibody detects transfected levels of total gremlin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Val170 of human gremlin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Gremlin is a secreted antagonist of bone morphogenetic proteins that play important roles during development. Gene targeting generates gremlin deficient mice that die shortly after birth due to absent kidneys and defects in lung septation (1-3). Gremlin also plays a role in cardiomyogenesis and angiogenesis (4,5). TGF-β can induce gremlin expression under pathological conditions. Gremlin colocalizes with TGF-β in tubulointerstitial fibrosis associated with chronic allograft nephropathy and is expressed in diabetic nephropathy in vivo (6-8). Gremlin is widely expressed in cancer associated stromal cells and can enhance cancer cell proliferation (9).

1. Zúñiga, A. et al. (1999) Nature 401, 598-602.
2. Zuniga, A. et al. (2004) Genes Dev 18, 1553-64.
3. Michos, O. et al. (2004) Development 131, 3401-10.
4. Stabile, H. et al. (2007) Blood 109, 1834-40.
5. Kami, D. et al. (2008) PLoS ONE 3, e2407.
6. Carvajal, G. et al. (2008) Transplant Proc 40, 734-9.
7. McMahon, R. et al. (2000) J Biol Chem 275, 9901-4.
8. Dolan, V. et al. (2005) Am J Kidney Dis 45, 1034-9.
9. Sneddon, J.B. et al. (2006) Proc Natl Acad Sci USA 103, 14842-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.