NeuroD (D35G2) Rabbit mAb

• 产品编号：CST-4373S      品牌：CST       原厂货号：4373S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NeuroD (D35G2) Rabbit mAb 兔单抗识别内源性的总NeuroD蛋白。该单克隆抗体由合成的人类NeuroD蛋白Gln15位点附近肽段免疫动物而制成。NeuroD是基本的螺旋 - 环 - 螺旋（bHLH）转录因子家族成员。这些蛋白质与E蛋白形成异二聚体，结合经典的E盒序列CANNTG（1,2）。CaMKII介导NeuroD的Ser336磷酸化，这是树突形成和增长所必需的（3,4）。NeuroD的Ser274也可磷酸化，其结果根据磷酸化的酶不同而不同，胰腺β细胞中ERK的磷酸化促进NeuroD活性，神经元中GSK-3β的磷酸化抑制NeuroD活性（3）。NeuroD在胰腺和发育中的神经系统是至关重要的，在内耳和哺乳动物的视网膜的发育中起着很大的作用（3）。缺乏NeuroD的小鼠会发生严重的糖尿病，因缺乏β细胞的分化而在出生不久即死亡（2,3,5,6）。脑内缺乏NeuroD将导致严重的发育障碍（5）。人类该基因突变与I型糖尿病和年轻成年发病的糖尿病有关（1,3）。

1. Schonhoff, S.E. et al. (2004) Endocrinology 145, 2639-2644.
2. Sharma, A. et al. (1999) Mol. Cell Biol. 19, 704-713.
3. Chae, J.H. et al. (2004) Mol. Cells 18, 271-288.
4. Gaudillière, B. et al. (2004) Neuron 41, 229-241.
5. Miyata, T. et al. (1999) Genes Dev. 13, 1647-1652.
6. Naya, F.J. et al. (1997) Genes Dev. 11, 2323-2334.

原厂资料：

Specificity / Sensitivity

NeuroD (D35G2) Rabbit mAb detects endogenous levels of total NeuroD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gln15 of human NeuroD protein.

Background

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG (1,2). Neuronal activity results in CaMKII-mediated phosphorylation of NeuroD at Ser336, which is necessary for formation and growth of dendrites (3,4). NeuroD is also phosphorylated at Ser274 though the results are context dependent as phosphorylation by Erk stimulates NeuroD activity in pancreatic β-cells while phosphorylation by GSK-3β inhibits NeuroD in neurons (3). NeuroD is crucially important in both the pancreas and developing nervous system, and plays a large role in the development of the inner ear and mammalian retina (3). Mice lacking NeuroD become severely diabetic and die shortly after birth due to defects in β-cell differentiation (2,3,5,6). The lack of NeuroD in the brain results in severe defects in development (5). Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young (1,3).

1. Schonhoff, S.E. et al. (2004) Endocrinology 145, 2639-2644.
2. Sharma, A. et al. (1999) Mol. Cell Biol. 19, 704-713.
3. Chae, J.H. et al. (2004) Mol. Cells 18, 271-288.
4. Gaudillière, B. et al. (2004) Neuron 41, 229-241.
5. Miyata, T. et al. (1999) Genes Dev. 13, 1647-1652.
6. Naya, F.J. et al. (1997) Genes Dev. 11, 2323-2334.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.