UGT Antibody

• 产品编号：CST-4371S      品牌：CST       原厂货号：4371S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

UGT Antibody用于检测内源性UGT1A亚家族。多克隆抗体通过用对应于人源UGT1A蛋白羧基序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。葡萄糖醛酸化反应是体内的一个重要的生理过程，此反应加速将亲脂性的内源和外源物质的清除过程，将其转化成亲水物质分泌到胞外（1,2）。尿苷二磷酸葡萄糖醛酸基转移酶（UGT）超家族催化核糖糖基的醛酸化反应，将其转变成多种内源及外源的化合物。超过100个UGT哺乳动物基因被相继阐述，并且根据它们的序列同源性被划分成了多个亚家族（3）。 UGT1家族包含几种由可变剪切产生的基因产物。不同的UGT有不同的组织特异性表达和底物特异性。同时，不同个体的UGT同工酶的不同表达与个体间药物代谢差异有关。

1. Wells, P.G. et al. (2004) Drug Metab. Dispos. 32, 281-90.
2. King, C.D. et al. (2000) Curr. Drug Metab. 1, 143-61.
3. Mackenzie, P.I. et al. (2005) Pharmacogenet. Genomics 15, 677-85.

原厂资料：

Specificity / Sensitivity

UGT Antibody detects endogenous levels of proteins from the UGT1A subfamily.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of the UGT1A subfamily. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Glucuronidation is a major pathway that enhances the elimination of lipophilic xenobiotics and endobiotics to more more water soluble compounds for excretion (1,2). The UDP-glucuronosyltransferase (UGT) superfamily catalyzes the glucuronidation of the glycosyl group of a nucleotide sugar to a variety of endogenous and exogenous compounds. Over 100 UGT mammalian gene products have been described and have been divided into subfamilies based on sequence identities (3). The UGT1 subfamily consists of a number of gene products resulting from alternative splicing. These UGT products can differ in tissue expression and substrate specificity. Also, marked differences in the individual expression of UGT isoforms can account for differences in drug metabolism.

1. Wells, P.G. et al. (2004) Drug Metab. Dispos. 32, 281-90.
2. King, C.D. et al. (2000) Curr. Drug Metab. 1, 143-61.
3. Mackenzie, P.I. et al. (2005) Pharmacogenet. Genomics 15, 677-85.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody