IRAK-M Antibody

• 产品编号：CST-4369S      品牌：CST       原厂货号：4369S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRAK-M抗体能够检测内源性IRAK-M蛋白总体水平。 此抗体不与其它家族成员交叉反应。此多克隆抗体是通过合成人源对应的IRAK-M C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。细胞白介素1(IL-1)受体相关激酶(IRAK)是丝氨酸/酪氨酸特异性激酶，它通过IL-1诱导的方式与IL-1受体发生共沉淀(1)。 哺乳动物的IRAK家族有四个成员(IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4)。IL-1结合到IL-1的I类受体(IL-1RI)而促进形成包含IL-1RI, AcP, MyD88和IRAKs的复合体(2)。IRAK在IL-1刺激后能够在短时间内自磷酸化。接着IRAK从IL-1RI复合体上解离，泛素化并与两个膜结合蛋白 TAB2 和TRAF6结合。IRAK-TRAF6-TAB2复合体释放到细胞质中并激活蛋白激酶的级联反应，包括TAK1、IKKs 和应激活化激酶 (3)。与IRAK1和 IRAK4不同, IRAK2 和IRAK-M并不具有重要的激酶活性，尽管他们在过表达后也能激活NF-κB(4)。与家族中的其它成员相比，IRAK-M的表达更为局限，只是在单核细胞/巨噬细胞中高表达(4)。研究IRAK-M敲除小鼠表明，IRAK-M在Toll样受体信号通路和天然免疫应答中作为负调控因子，它们是通过阻止IRAK1和IRAK4与MyD88的解体并接下来与TRAF6形成复合体而实现上述功能(5)。

1. Dinarello, C.A. (1996) Blood 87, 2095-147.
2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
4. Wesche, H. et al. (1999) J. Biol. Chem. 274, 19403-10.
5. Kobayashi, K. et al. (2002) Cell 110, 191-202.

原厂资料：

Specificity / Sensitivity

IRAK-M antibody detects endogenous levels of total IRAK-M protein. Cross reactivity was not detected with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human IRAK-M. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3).Unlike IRAK1 and IRAK4, IRAK2 and IRAK-M do not have significant kinase activity although they can still activate NF-κB when overexpressed (4). Expression of IRAK-M is more restricted compared to other family members with highest levels of expression occurring in monocytes/macrophages (4). Studies from IRAK-M knockout mice suggest that IRAK-M may play a role as a negative regulator of Toll-like receptor signaling and innate immune responses by preventing the dissociation of IRAK1 and IRAK4 from MyD88 and the subsequent formation of its complex with TRAF6 (5).

1. Dinarello, C.A. (1996) Blood 87, 2095-147.
2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
4. Wesche, H. et al. (1999) J. Biol. Chem. 274, 19403-10.
5. Kobayashi, K. et al. (2002) Cell 110, 191-202.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.