The purified antibody is not directed against any known antigen. It functions as an isotype control for rabbit antibodies.
Source / Purification
The antibody was isolated from naive rabbit sera and prepared by affinity chromotography. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with a F/P ratio of 2-6.
Description
Rabbit IgG was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis of human and mouse cells.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
注意事项:
Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze