GSK-3α (D80E6) Rabbit mAb

产品编号：CST-4337S 品牌：CST 原厂货号：4337S
产品分类：抗体 > 一抗 > 蛋白特异性一抗
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包装：

运保温度:

–20°C

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描述：

GSK-3α (D80E6) Rabbit mAb兔单抗可以检测内源性总GSK-3蛋白，不和GSK-3β发生交叉反应。该单克隆抗体是采用合成的与人源GSK-3α蛋白序列相对应的肽段免疫动物生产的。最初人们认为糖原合酶激酶-3（GSK-3）在胰岛素的刺激下参与调节糖原合成（1）。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶，可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3 Kinase/ Akt细胞存活通路上的一个关键下游分子，其活性可以被Akt介导的GSK-3α的Ser21和GSK-3β的Ser9磷酸化抑制（2,3）。GSK-3不仅参与盘基网柄菌细胞命运调控，而且是果蝇，爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分（4）。GSK-3还参与调节cyclin D1 蛋白裂解和亚细胞定位（5）。 GSK-3a 调节β淀粉样肽的合成，后者是在阿尔兹海默症发展过程中积累的斑块的主要成分。给予治疗剂量的锂---一种GSK-3抑制剂，可以减少β淀粉样肽的产生，因此阻断了含有过量APP的小鼠大脑中淀粉样-β肽的积累。而这是通过特异性的抑制由γ-分泌酶介导的淀粉样前蛋白（APP）的剪切来实现的（6）。

原厂资料：

Specificity / Sensitivity

GSK-3α (D80E6) Rabbit mAb detects endogenous levels of total GSK-3α protein. The antibody does not cross-react with GSK-3β.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GSK-3α protein.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3α regulates the production of amyloid-β peptides, a major component of the plaques that accumulate with progression of Alzheimer disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-β production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by γ-secretase, blocking accumulation of amyloid-β peptides in the brains of mice that overproduce APP (6).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody