Rab25 Antibody

• 产品编号：CST-4314S      品牌：CST       原厂货号：4314S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab25 Antibody识别内源性的Rab25总蛋白。该多克隆抗体用与人类Rab25中Ile185位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。Rab11a、Rab11b和Rab25是小Ras样GTP酶的Rab11亚家族成员。与广泛表达的Rab11不同，典型的Rab25的表达似乎局限于胃肠道粘膜、肾脏和肺（1）。Rab25可以与顶端循环囊泡结合，帮助顶端囊泡转运（2,3）。非典型Rab25的表达已经与多种癌症相关联。增加Rab25的表达与卵巢和乳腺癌的侵袭性生长有关，因为Rab25可能通过调节囊泡转运和细胞运动来抑制凋亡和促进癌症细胞增生和侵袭（4-7）。Rab25和β1整合素的相互作用促进囊泡介导的整合素转运至伪足端膜，促进肿瘤细胞的持续浸润（8）。相反的，报道的Rab25在一些乳腺癌病例中的表达缺失存在着其对癌症的发生尚不清楚的作用（9）。

1. Goldenring, J.R. et al. (1993) J Biol Chem 268, 18419-22.
2. Casanova, J.E. et al. (1999) Mol Biol Cell 10, 47-61.
3. Wang, X. et al. (2000) J Biol Chem 275, 29138-46.
4. Cheng, K.W. et al. (2004) Nat Med 10, 1251-6.
5. Cheng, K.W. et al. (2005) Cancer Res 65, 2516-9.
6. Chia, W.J. and Tang, B.L. (2009) Biochim Biophys Acta 1795, 110-6.
7. Tang, B.L. and Ng, E.L. (2009) Cell Motil Cytoskeleton 66, 365-70.
8. Caswell, P.T. et al. (2007) Dev Cell 13, 496-510.
9. Cheng, J.M. et al. (2010) Int J Cancer 126, 2799-812.

原厂资料：

Specificity / Sensitivity

Rab25 Antibody detects endogenous levels of total Rab25 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile185 of human Rab25 protein.

Background

Rab11a, Rab11b and Rab25 are members of the Rab11 subfamily of small Ras-like GTPases. Unlike universally expressed Rab11, typical Rab25 expression appears to be limited to gastrointestinal mucosa, kidney, and lung (1). Rab25 can associate with apical recycling vesicles to help regulate apical vesicle trafficking (2,3). Atypical Rab25 expression has been associated with various forms of cancer. Increased Rab25 expression is associated with aggressive growth in ovarian and breast cancer, where Rab25 may inhibit apoptosis and promote cancer cell proliferation and invasion through regulation of vesicle transport and cellular motility (4-7). Interaction between Rab25 and β1 integrin promotes vesicle-mediated transport of integrin to pseudopodial tip membranes, fostering the persistent invasion of tumor cells (8). Conversely, the reported loss of Rab25 expression in a number of breast cancer cases has an unclear effect on cancer pathogenesis (9).

1. Goldenring, J.R. et al. (1993) J Biol Chem 268, 18419-22.
2. Casanova, J.E. et al. (1999) Mol Biol Cell 10, 47-61.
3. Wang, X. et al. (2000) J Biol Chem 275, 29138-46.
4. Cheng, K.W. et al. (2004) Nat Med 10, 1251-6.
5. Cheng, K.W. et al. (2005) Cancer Res 65, 2516-9.
6. Chia, W.J. and Tang, B.L. (2009) Biochim Biophys Acta 1795, 110-6.
7. Tang, B.L. and Ng, E.L. (2009) Cell Motil Cytoskeleton 66, 365-70.
8. Caswell, P.T. et al. (2007) Dev Cell 13, 496-510.
9. Cheng, J.M. et al. (2010) Int J Cancer 126, 2799-812.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.