p47phox Antibody

• 产品编号：CST-4312S      品牌：CST       原厂货号：4312S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

p47phox Antibody能够检测内源p47phox蛋白总体水平。此多克隆抗体是通过合成人源对应的p47phox Arg292位点周围的肽段来免疫动物而获得。抗体是通过多肽亲和层析纯化。噬菌细胞的NADPH氧化酶是多蛋白复合酶，在病原菌侵入时它能催化还原氧成为超氧化物。NADPH 氧化酶包含6个亚单元, 包括膜结合 p91 phox和 p22 phox 异二聚体 (也叫做细胞色素 b558), 细胞质复合体p40phox, p47phox 和 p67phox, 还有小GTPase Rac2。NADPH氧化酶的激活是从细胞质复合体的磷酸化开始, 这就诱导了构象的变化，并导致了细胞质复合体定位到膜上，从而形成具有活性的与细胞色素b558结合的酶 (1)。p47phox的缺失, 通常导致了p47phox和邻近同源假基因的重组，引起慢性肉芽肿病(2-4)。由于心肌的NADPH氧化酶活性的增加导致了氧化应激升高可能是心衰竭的因素之一(5,6)。

1. Babior, B.M. (1999) Blood 93, 1464-76.
2. Noack, D. et al. (2001) Blood 97, 305-11.
3. Görlach, A. et al. (1997) J Clin Invest 100, 1907-18.
4. Chanock, S.J. et al. (2000) Blood Cells Mol Dis 26, 37-46.
5. Heymes, C. et al. (2003) J Am Coll Cardiol 41, 2164-71.
6. Doerries, C. et al. (2007) Circ Res 100, 894-903.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

p47phox Antibody detects endogenous levels of total p47phox protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg292 of human p47phox. Antibodies are purified by peptide affinity chromatography.

Background

The phagocytic NADPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The NADPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of NADPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformational change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558 (1). Defects in p47phox, often resulting from recombination between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease (2-4). Elevated oxidative stress due to increased myocardial NADPH oxidase activity may be a contributing factor in heart failure (5,6).

1. Babior, B.M. (1999) Blood 93, 1464-76.
2. Noack, D. et al. (2001) Blood 97, 305-11.
3. Görlach, A. et al. (1997) J Clin Invest 100, 1907-18.
4. Chanock, S.J. et al. (2000) Blood Cells Mol Dis 26, 37-46.
5. Heymes, C. et al. (2003) J Am Coll Cardiol 41, 2164-71.
6. Doerries, C. et al. (2007) Circ Res 100, 894-903.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody