Thymidine Phosphorylase/ECGF1 (D69B12) Rabbit mAb

• 产品编号：CST-4307S      品牌：CST       原厂货号：4307S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Thymidine Phosphorylase/ECGF1 (D69B12) Rabbit mAb兔单抗能够检测内源性水平TP/ECGF1总蛋白。该单克隆抗体是采用合成的人源thymidine phosphorylase/ECGF1蛋白Gln370周围残基相对应的肽段免疫动物而制备的。

胸苷磷酸化酶（TP）是血小板衍生的内皮细胞生长因子（PD-ECGF），能够催化胸苷和正磷酸盐形成胸腺嘧啶和2-脱氧-D-核糖-1-磷酸（1）。这种胞内酶能促进血管生成和抑制细胞凋亡。TP的催化活性对于其血管生成功能是必需的（2,3）。在各种各样不同的实体瘤和炎症性疾病中，经常会观察到TP/PD-ECGF表达量的增加，这往往与预后不良相关（4,5）。另外，TP可以激活氟尿嘧啶衍生物（DFUR）前体药物，增加相关治疗的抗肿瘤活性（1,5）。人们已经广泛开展胸苷磷酸作为癌症治疗靶点的应用研究，重点在于抑制TP酶的活性或在伴DFUR治疗中增加酶的活性（1,5）。

1. Rooseboom, M. et al. (2004) Pharmacol Rev 56, 53-102.
2. Moghaddam, A. and Bicknell, R. (1992) Biochemistry 31, 12141-6.
3. Furukawa, T. et al. (1992) Nature 356, 668.
4. Toi, M. et al. (2005) Lancet Oncol 6, 158-66.
5. Liekens, S. et al. (2007) Biochem Pharmacol 74, 1555-67.

原厂资料：

Specificity / Sensitivity

Thymidine Phosphorylase/ECGF1 (D69B12) Rabbit mAb detects endogenous levels of total TP/ECGF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln370 of human thymidine phosphorylase/ECGF1 protein.

Background

Thymidine phosphorylase (TP) is a platelet-derived endothelial cell growth factor (PD-ECGF) that catalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate (1). This intracellular enzyme is capable of both promoting angiogenesis and inhibiting apoptosis. Thymidine phosphorylase catalytic activity is required for its angiogenic function (2,3). Increased expression of TP/PD-ECGF is seen in a wide variety of different solid tumors and inflammatory diseases and is often associated with poor prognosis (4,5). Alternatively, TP can activate fluorouracil derivative (DFUR) prodrugs and increase the antitumor activity of the related treatment (1,5). The use of thymidine phosphorylase as a cancer therapeutic target has been studied extensively, with emphasis on either inhibiting TP enzymatic activity or increasing enzyme induction with concomitant DFUR treatment (1,5).

1. Rooseboom, M. et al. (2004) Pharmacol Rev 56, 53-102.
2. Moghaddam, A. and Bicknell, R. (1992) Biochemistry 31, 12141-6.
3. Furukawa, T. et al. (1992) Nature 356, 668.
4. Toi, M. et al. (2005) Lancet Oncol 6, 158-66.
5. Liekens, S. et al. (2007) Biochem Pharmacol 74, 1555-67.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than
0.02% sodium azide. Store at –20°C. Do not aliquot the
antibody